Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,525,336	(G)5→6	coding (645/1500 nt)	ansP ←	L‑asparagine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,525,331	1	.	G	94.0%	164.1 / 2.9	50	coding (650/1500 nt)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (29/18);  total (31/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGT‑G‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCG  >  NC_000913/1525206‑1525460                                                                                                                               |                                                                                                                                     tGTTAATGGCTTTAGGCCCCATGGTCTGCGGATCTTTACATACTCCGGCAGCTGGACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCGGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGATgcc                                                                                                                      <  2:47605/140‑1 (MQ=255)   ttAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAgccgc                                                                                                                    >  1:53439/1‑140 (MQ=255)   ttAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAgccgc                                                                                                                    >  1:374560/1‑140 (MQ=255)     aaTGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTCCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCa                                                                                                                  >  1:606473/1‑140 (MQ=255)         gCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGTCATTAt                                                                                                              <  1:132168/140‑1 (MQ=255)           tttAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCg                                                                                                            >  2:119735/1‑140 (MQ=255)           tttAGGCACCATGGTATGCGGACCTTTACTTTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCg                                                                                                            <  1:305161/140‑1 (MQ=255)             tAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGt                                                                                                          >  1:441352/1‑140 (MQ=255)                    cATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCTCAGGCAGCGAACAGA‑GTGG‑G‑GAAGAAGCCGTTATTATCGGTGATTaaa                                                                                                   >  2:305161/1‑140 (MQ=255)                     aTGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAAt                                                                                                  >  2:68662/1‑140 (MQ=255)                       ggTCTGCGGATCTTTCCATTCTTCGACAGCTGTACCCCCCA‑TTCAATGGAGGCAAAAGCGAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGaa                                                                                               <  1:195429/140‑1 (MQ=255)                       ggTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCA‑CAATTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGaa                                                                                               <  1:151261/140‑1 (MQ=255)                        gtttGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGaa                                                                                               <  1:155528/137‑1 (MQ=255)                         tCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGaaa                                                                                              >  2:454212/1‑140 (MQ=255)                                   tttACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAgtggtg                                                                                    <  1:422159/140‑1 (MQ=255)                                            tcCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATcc                                                                           >  1:41638/1‑140 (MQ=255)                                            tcCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATcc                                                                           >  1:604622/1‑140 (MQ=255)                                            tcCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATcc                                                                           >  1:575628/1‑140 (MQ=255)                                              cGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAg                                                                         >  2:333150/1‑140 (MQ=255)                                                      gtccccACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGAc                                                                 <  2:630907/137‑1 (MQ=255)                                                           caccaTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCTAGCGGCTGACCACTa                                                            >  1:268556/1‑140 (MQ=255)                                                            cccaTTTCAATGGAGGCAAAAGCAATCACTACGCCCTTAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTAc                                                           <  1:287284/139‑1 (MQ=255)                                                             ccaTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTAcc                                                          <  1:78080/140‑1 (MQ=255)                                                             ccaTTTCAATGGAGGAAAAAGCTAATACTGCGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTAcc                                                          <  1:245432/140‑1 (MQ=255)                                                              caTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCg                                                         >  2:348302/1‑140 (MQ=255)                                                              caTTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCg                                                         >  2:30874/1‑140 (MQ=255)                                                                 ttCAATGGAGGCAAAGGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAgg                                                      >  1:530386/1‑140 (MQ=255)                                                                 ttCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAgg                                                      <  2:604622/140‑1 (MQ=255)                                                                   cAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGaa                                                    <  1:462111/140‑1 (MQ=255)                                                                         aGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGt                                              >  2:409552/1‑140 (MQ=255)                                                                           gCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTAc                                            >  2:488005/1‑140 (MQ=255)                                                                           gCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTAc                                            >  2:504384/1‑140 (MQ=255)                                                                           gCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTAc                                            >  2:428557/1‑140 (MQ=255)                                                                           gCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAACGTATGACCACTATCGAGGAACACTGTAc                                            >  1:272636/1‑140 (MQ=255)                                                                           gCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTACCATCCACCGGTTGACCACTATCGAGGAACACTGTAc                                            >  1:239538/1‑140 (MQ=255)                                                                           gCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGT‑G‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTAcc                                           >  1:499950/1‑140 (MQ=255)                                                                            cAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATAAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTAcc                                           <  2:441352/140‑1 (MQ=255)                                                                                     acacTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCaa                                  <  1:68662/140‑1 (MQ=255)                                                                                     acacTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCaa                                  >  1:408691/1‑140 (MQ=255)                                                                                           cGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGt                            >  1:453418/1‑140 (MQ=255)                                                                                            gCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTc                           >  1:515157/1‑140 (MQ=255)                                                                                                tGAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACaa                       >  2:334994/1‑140 (MQ=255)                                                                                                 gAATCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAAt                      >  1:545372/1‑140 (MQ=255)                                                                                                    tCAACACCAGCGCAGGCCGCAATCCGT‑G‑GG‑GTGAACAAGCCGCCATTAGCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGc                   <  2:192817/140‑1 (MQ=255)                                                                                                    tCAACACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGc                   <  2:408691/140‑1 (MQ=255)                                                                                                    acAACACCTGCGCAGGCGGCAACCCGT‑G‑GGAC‑GCACAAGGCGCCATTAGCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGc                   <  2:309480/139‑1 (MQ=255)                                                                                                      aaCACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGa                 <  2:515157/140‑1 (MQ=255)                                                                                                      aaCACCAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGa                 <  2:53439/140‑1 (MQ=255)                                                                                                          ccAGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCAc             >  1:383772/1‑140 (MQ=255)                                                                                                            aGCGCAGGCAGCAAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACtt           >  1:105189/1‑140 (MQ=255)                                                                                                                     agcaAACCGTGG‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTGACGATGGCGTGCACTTTAATAAgcg  >  1:308789/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                     TGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGT‑G‑GG‑G‑GAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCACTACCGAGGAACACTGTACCCACGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCG  >  NC_000913/1525206‑1525460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑