Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,597,313 (C)7→8 pseudogene (774/5422 nt) yneO ← AIDA‑I family autotransporter YneO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,597,3061.C87.0% 138.5 / 14.1 46pseudogene (781/5422 nt)yneOAIDA‑I family autotransporter YneO
Reads supporting (aligned to +/- strand):  ref base . (2/4);  new base C (23/17);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

TTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAA‑CCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGA  >  NC_000913/1597167‑1597436
                                                                                                                                            |                                                                                                                                   
ttGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaaccaa                                                                                                                                      >  2:475153/1‑140 (MQ=255)
    aaaTATTGACTGTGCCAGTACCAAAATATCCCTTATAGAATCTGCGTATCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAA‑cccc                                                                                                                                 <  2:226557/140‑1 (MQ=255)
     aaTATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAA‑ccccc                                                                                                                                >  1:588076/1‑140 (MQ=255)
     aaTATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAA‑ccccc                                                                                                                                <  1:478104/140‑1 (MQ=255)
       tatTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAA‑cccccc‑c                                                                                                                             <  2:165038/140‑1 (MQ=255)
               gtgCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGACACCGGTGGCATTGCCACCGATAGTCGTATTCTCAGCCATAACCAACCCCCCC‑CTCGCGaa                                                                                                                      >  1:298116/1‑140 (MQ=255)
                tgCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAAt                                                                                                                     >  1:341655/1‑140 (MQ=255)
                tgCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAAt                                                                                                                     >  1:539322/1‑140 (MQ=255)
                tgCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAA‑CCCCCC‑CTCGCGAATa                                                                                                                    <  2:528125/140‑1 (MQ=255)
                 gCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATa                                                                                                                    >  2:12070/1‑140 (MQ=255)
                 gCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATa                                                                                                                    <  2:613878/140‑1 (MQ=255)
                 gCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATa                                                                                                                    >  2:83067/1‑140 (MQ=255)
                      tACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGc                                                                                                               <  2:368936/140‑1 (MQ=255)
                      tACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGc                                                                                                               <  1:12070/140‑1 (MQ=255)
                      tACCGAAATATCCATAATAGAGCCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGc                                                                                                               <  1:225465/140‑1 (MQ=255)
                          gAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAC‑CCCCCC‑CTCGCGAATAGTCGCCTccc                                                                                                          >  1:43193/1‑140 (MQ=255)
                          gAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAC‑CCCCCC‑CTCGCGAATAGTCGCCTccc                                                                                                          <  2:43193/140‑1 (MQ=255)
                            aaTATCCATTATAGAGTTTGCGGACCGTGATGACAGAGTTTTGATCCACCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTcccc                                                                                                         <  2:236504/140‑1 (MQ=255)
                             atatCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCa                                                                                                        <  2:61687/140‑1 (MQ=255)
                               atCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGt                                                                                                      <  1:83067/140‑1 (MQ=255)
                                     tataGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTg                                                                                                >  2:5193/1‑140 (MQ=255)
                                          agTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTAc                                                                                           >  1:414971/1‑140 (MQ=255)
                                            tCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCa                                                                                         >  1:649349/1‑140 (MQ=255)
                                            tCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCa                                                                                         >  1:88603/1‑140 (MQ=255)
                                              tGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAAt                                                                                       >  2:343952/1‑140 (MQ=255)
                                                 gTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCCCGGATAGTCGCCTCCTCAGTTCCTTGATTACCAATTTg                                                                                    >  2:266511/1‑140 (MQ=255)
                                                      gTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAAtt                                                                               <  2:120447/140‑1 (MQ=255)
                                                          tgaCAGAGTCTTGATCCTGCACATTCAGGGGTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGACATTCAAt                                                                           >  1:374861/1‑140 (MQ=255)
                                                              agagTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAg                                                                       >  2:38647/1‑140 (MQ=255)
                                                                 gTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCACGTTCCTAGATTACGAATTTGAAATTCAATTGAGGaa                                                                    >  1:247031/1‑140 (MQ=255)
                                                                  tCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCTGTTCCTTGATTACCTATTTGATATTCAATTGAGGAAt                                                                   >  1:167980/1‑140 (MQ=255)
                                                                    ttGATCCTGCACATTGAGGGTTCCGTTACCGGTGGCAATGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCa                                                                 <  1:303695/140‑1 (MQ=255)
                                                                          cTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTAttt                                                           >  1:658065/1‑140 (MQ=255)
                                                                          cTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTAttt                                                           >  2:94325/1‑140 (MQ=255)
                                                                            gCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTAttttt                                                         >  2:429515/1‑140 (MQ=255)
                                                                            gCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTAttttt                                                         >  2:95288/1‑140 (MQ=255)
                                                                                  tCAGGGTTCCGATACCGGTGGCTTTGCCACCGAGAGTCGCATTTTCAGGCGTAACCAA‑CCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGc                                                  <  2:297915/140‑1 (MQ=255)
                                                                                  tCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGGTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAg                                                   >  2:646576/1‑140 (MQ=255)
                                                                                            gATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAACTCAATTGGGGAATCATAATTTTTTATTAGCCACTCGCCa                                         >  2:209065/1‑140 (MQ=255)
                                                                                                     ggCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCa                                >  1:461771/1‑140 (MQ=255)
                                                                                                        aTTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACg                             <  2:461771/140‑1 (MQ=255)
                                                                                                         ttGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGa                            <  2:539322/140‑1 (MQ=255)
                                                                                                             cACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAAc                        <  1:57303/140‑1 (MQ=255)
                                                                                                             cACCGATAATCGTATGTTCAGCTGTGACCTA‑CCCCCCACTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAAc                        <  1:212024/140‑1 (MQ=255)
                                                                                                                  aTAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCt                      <  2:385025/137‑1 (MQ=255)
                                                                                                                  aTAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCt                      >  1:385025/1‑137 (MQ=255)
                                                                                                                  aTAATCGTATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGc                   >  1:434169/1‑140 (MQ=255)
                                                                                                                         tATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACt            <  2:414971/140‑1 (MQ=255)
                                                                                                                         tATTTTCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACt            <  1:94325/140‑1 (MQ=255)
                                                                                                                            tttCAGCCGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGcc         <  2:359566/140‑1 (MQ=255)
                                                                                                                            tttCAGCCGTAACCAA‑CCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGccc        >  2:585681/1‑140 (MQ=255)
                                                                                                                                   cGTAACCAACCCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGa  <  2:535050/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                   
TTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAA‑CCCCCC‑CTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCCATTACTGCCCGCCTGA  >  NC_000913/1597167‑1597436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑