| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,765,674 | (C)5→6 | coding (3012/3057 nt) | ydiJ ← | putative FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,765,669 | 1 | . | C | 92.9% | 88.6 / 0.6 | 28 | coding (3017/3057 nt) | ydiJ | putative FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (14/12); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTA‑CCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAATTTTCATGGTTT > NC_000913/1765532‑1765800 | tcatcaCCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCAATTAATAAGCTCCAGTAAAGCCTGCACAGGATGGCGTA‑cc > 2:237478/1‑140 (MQ=255) cAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATGTCCAGTAAAGCCTGCACAGGATGGCGTA‑cccc‑ccgtg > 1:254538/1‑136 (MQ=255) cAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGc > 1:113824/1‑140 (MQ=255) ccTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGt > 1:115156/1‑140 (MQ=255) tCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTA‑CCCCAC‑GTGCCTTTAACGCGTGTTACCTGGATACGGCAGGAATATCCGGTCGc > 2:257939/1‑140 (MQ=255) tAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAg > 1:40110/1‑140 (MQ=255) cTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGc > 2:161063/1‑140 (MQ=255) ttCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCa > 2:31311/1‑140 (MQ=255) gggTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTTGc > 2:117372/1‑140 (MQ=255) gTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTg > 2:612992/1‑140 (MQ=255) ttCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGc > 1:60217/1‑140 (MQ=255) tCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTTCGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGcc < 1:663661/140‑1 (MQ=255) ccGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCt < 2:115156/140‑1 (MQ=255) tCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGc < 2:406617/140‑1 (MQ=255) tCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGc < 1:31311/140‑1 (MQ=255) tatCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACGGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAgg < 2:254538/140‑1 (MQ=255) tatCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAgg < 2:162776/140‑1 (MQ=255) atCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGa < 2:40110/140‑1 (MQ=255) tCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTTGCTGCATAGCCTGATGCCAGGAt < 1:117372/140‑1 (MQ=255) tCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGAt < 1:443705/140‑1 (MQ=255) tCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGAt < 2:113824/140‑1 (MQ=255) aTTTAATAATCTCCAGTAAAGCCTGAACATGATGGCGCACCCCC‑C‑GTGCCTTCAACCCATTTTACCTGGCTACGGCAGGAATATTCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATaa < 1:237478/140‑1 (MQ=255) tttAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAAc > 1:5780/1‑140 (MQ=255) tcCAGTAAAGCCTGCACAGGATGGCGTA‑CCCC‑CGGTGCTTTGACCCCCTTTTACCTGCCAACGGCAGGAAGTTCCGGCCGCCAGAAACCGGTTAGGCGCAAGACGTTGCATGGCCT‑TTTCACGAATAACTTATAGATccc > 2:444977/1‑140 (MQ=255) aaGCCTGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGa < 1:386168/140‑1 (MQ=255) tGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAAtttt > 2:329179/1‑140 (MQ=255) tGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAAtttt > 2:534061/1‑140 (MQ=255) tGCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACACCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAAtttt > 1:606815/1‑140 (MQ=255) gCACAGGATGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAATTTTc > 1:559061/1‑140 (MQ=255) aTGGCGTACCCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATCGAGCCCGAGTGAATTTTCATGGttt > 1:312025/1‑140 (MQ=255) | TCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTA‑CCCC‑C‑GTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAATTTTCATGGTTT > NC_000913/1765532‑1765800 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |