| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 144,977 | (A)7→8 | coding (401/441 nt) | yadI → | putative PTS enzyme IIA component YadI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 144,970 | 1 | . | A | 84.9% | 94.6 / 10.6 | 33 | coding (394/441 nt) | yadI | putative PTS enzyme IIA component YadI |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base A (15/13); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑C‑AAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTACAAACAAGCTG > NC_000913/144836‑145096 | aaGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑C‑aaa‑aa > 1:590531/1‑140 (MQ=255) aTTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑C‑aaa‑aaa‑aatc < 2:83834/140‑4 (MQ=255) tgtCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTc > 1:163524/1‑140 (MQ=255) tgtCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGACGGAGGTGTGTAGTCTATGGCACCACCAACC‑AAA‑AAA‑ATCCGCCTTTc > 2:197610/1‑140 (MQ=255) tCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAATCTTTGGAACCGACCA‑CAAAA‑AAA‑ATCCGCCTTTCGt > 1:266592/1‑140 (MQ=255) aCCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCaaa > 2:627591/1‑140 (MQ=255) aaCAGATGATGGCTTCCCGTGAAACCATGTCCAGTTCCGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTa < 1:191074/140‑1 (MQ=255) aCAGATGATGGCTTGCCGTGAATCCATGACCAGTTCATAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTAt < 1:242776/140‑1 (MQ=255) aCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTAt < 2:590531/140‑1 (MQ=255) aCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTAt < 1:627591/140‑1 (MQ=255) atgatgGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGt > 2:91654/1‑140 (MQ=255) gCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCcgc > 2:405952/1‑140 (MQ=255) gCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTGTCGTCCTGAAAAATTATTTGTATGAGTATTAACCcgc > 1:172803/1‑140 (MQ=255) gCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑C‑AAATAAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCcgc > 1:487417/1‑140 (MQ=255) cGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGa > 1:497805/1‑140 (MQ=255) ccATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGAt < 1:528238/140‑1 (MQ=255) cATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATg < 1:91654/140‑1 (MQ=255) tGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGgc < 2:163524/140‑1 (MQ=255) ttCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTg > 2:546056/1‑140 (MQ=255) cgcgAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATaaa > 2:449481/1‑140 (MQ=255) cgcgAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATaaa > 2:436893/1‑140 (MQ=255) aGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTGCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCg < 1:152084/140‑1 (MQ=255) ttGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGttt < 1:537322/140‑1 (MQ=255) aCTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGACGGCGCTTGTGCCACGATAAAAGCGTTGTTTCACCCTc > 1:321834/1‑140 (MQ=255) ccGGAGGTGAGTAGTCTTTGGCCCCCACAC‑C‑AAA‑AAATATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTAttttt < 1:197610/140‑1 (MQ=255) aGGTGAGTAGTCTTTGGCACCAACAA‑C‑AAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTCCACCTTCGGTTATTTTTTCATg > 2:190280/1‑140 (MQ=255) ggTGAGTAGTCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATg < 2:497805/140‑1 (MQ=255) gtCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTacaaaca < 1:436893/140‑1 (MQ=255) gtCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTacaaaca < 1:449481/140‑1 (MQ=255) tCTTTGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTacaaacaa > 1:530579/1‑140 (MQ=255) tttGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTACAAACAAGc < 1:541388/140‑1 (MQ=255) tttGGCACCAACAA‑C‑AAATAAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTACAAACAAGc < 2:487417/140‑1 (MQ=255) ttGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGGTGTTTCAGCCTCGGTTCTTTTTTCATGTACAAACAAGCt > 2:286910/1‑140 (MQ=255) tGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTACAAACAAGCTg > 1:475150/1‑140 (MQ=255) tGGCACCAACAA‑CAAAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCACGGGTATTTTTTCTTGTACAAACAAGCTg > 1:209096/1‑140 (MQ=255) | AAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAA‑C‑AAA‑AAA‑ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTACAAACAAGCTG > NC_000913/144836‑145096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |