| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,806,042 | (C)6→7 | coding (42/759 nt) | ydiY ← | acid‑inducible putative outer membrane protein YdiY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,806,036 | 1 | . | C | 96.4% | 92.4 / ‑3.3 | 28 | coding (48/759 nt) | ydiY | acid‑inducible putative outer membrane protein YdiY |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (16/11); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGA > NC_000913/1805904‑1806165 | gCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑‑c‑cccc‑cc > 2:99842/1‑139 (MQ=255) tGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCC‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCaa > 1:567869/1‑140 (MQ=255) cGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAg > 2:126887/1‑140 (MQ=255) gTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGc > 2:146025/1‑140 (MQ=255) tCAGATTACCTTCGAACTGTTTATTAGCGCCGGAAAGATAATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACCCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCtt < 2:320506/140‑1 (MQ=255) aGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCAAACATAATGATAGCAGGAACTGTCTTCAAAAGCTTCa > 1:220529/1‑140 (MQ=255) cTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCa < 1:99842/140‑1 (MQ=255) gAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAagag > 2:421729/1‑140 (MQ=255) aaaGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGTAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGt > 1:557315/1‑140 (MQ=255) ttttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTa > 1:592793/1‑140 (MQ=255) tttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTac < 2:511515/140‑1 (MQ=255) cTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATATCGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACaaaaaa > 2:410334/1‑140 (MQ=255) gCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAGCTGTCTTCTAAAACTTCATTTATCAGGAGTTGGTACAACAAAAAAaga > 2:325516/1‑140 (MQ=255) ccATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATcgcg > 1:547811/1‑140 (MQ=255) aGTAAAAACGGAATCATCGGCAGCTGCATTCAGGGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCgg > 2:46121/1‑140 (MQ=255) gTAAAAACGGAATCACCGGCAGATGCATTCAGTGACGAAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGa < 1:325516/140‑1 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGaa > 2:542394/1‑140 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGaa > 2:666490/1‑140 (MQ=255) tAAAAACGGAATCATCGGAAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGaa < 1:46121/140‑1 (MQ=255) aaaaaCGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGaaa > 1:419098/1‑140 (MQ=255) aaaaaCGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATGTATCAAGAATCCGTACGACAAAAAAGGAGACCATCGCGGTCCCGGaaa > 2:193712/1‑140 (MQ=255) aaaaCGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAAc > 1:345306/1‑140 (MQ=255) tcatcGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAg < 2:539212/140‑1 (MQ=255) cGGCAGCTGCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATc < 1:421729/140‑1 (MQ=255) gCATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTa < 1:598063/140‑1 (MQ=255) cATTCAGTGACGCAAACATGC‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAAC‑AAAAAAGAGACCATCGCGGTCCCGGAAACCTTCTTAAGGATCAAAGATTAGc < 1:126887/140‑1 (MQ=255) tCAGTGACGCAAACACG‑‑C‑CCCCTCGCCAACATAACGAGGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGt < 1:193712/140‑1 (MQ=255) cAGTGACGCAAACATG‑‑CACCCC‑GGCCAACATAACGGTGGCAGGAACTGACTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTc < 2:287634/140‑1 (MQ=255) aCGCAAACATGCCC‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTg < 2:567869/140‑1 (MQ=255) cGCAAACATGC‑C‑CCCC‑CGCCAATATCACGTTGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGa < 1:271010/140‑1 (MQ=255) | GCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑‑C‑CCCC‑CGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGA > NC_000913/1805904‑1806165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |