| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,818,550 | (C)7→8 | intergenic (‑50/+55) | chbF ← / ← chbR | monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,818,543 | 1 | . | C | 93.9% | 105.6 / 0.4 | 33 | intergenic (‑43/+62) | chbF/chbR | monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (17/14); total (19/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
GATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAAACG > NC_000913/1818416‑1818672 | gATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑cc‑ccc‑cccggac < 1:111228/140‑6 (MQ=255) ataaATCCTTCCAGTCACTCCGGGGCATATCCGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATcc < 1:277066/140‑1 (MQ=255) ataaATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATcc < 2:372946/140‑1 (MQ=255) tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAaataat > 2:10297/1‑129 (MQ=255) tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAaataat < 1:10297/129‑1 (MQ=255) tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCaa > 1:447463/1‑136 (MQ=255) tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCaa < 2:447463/136‑1 (MQ=255) tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAgg < 1:127970/140‑1 (MQ=255) tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAgg > 1:461031/1‑140 (MQ=255) tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCAGATCTG‑CCACCCGCCAGACATAGATAATCCAGCAACAg > 1:173522/1‑140 (MQ=255) ccAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGa < 2:361557/140‑1 (MQ=255) ccAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGa < 1:612064/140‑1 (MQ=255) aGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACa < 2:349954/140‑1 (MQ=255) aCTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGata < 2:461031/140‑1 (MQ=255) ccGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATAtgt < 2:53755/140‑1 (MQ=255) taGCTGCTCCCGCCACCGATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTc < 2:283180/140‑1 (MQ=255) aGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa > 1:390612/1‑140 (MQ=255) ctgctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CC‑CCC‑CCCGACATAAAAAAATCAACAACAAGGAAGATATGTGAATTTTCCggg > 2:667110/1‑139 (MQ=255) ctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGtat > 2:590268/1‑140 (MQ=255) cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATaa > 2:27696/1‑140 (MQ=255) acgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTaaa > 2:392586/1‑140 (MQ=255) ttttAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAg > 2:401007/1‑140 (MQ=255) tCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGa < 1:88327/140‑1 (MQ=255) ccTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGc > 2:373940/1‑140 (MQ=255) cAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTaa > 1:568079/1‑140 (MQ=255) aGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTaaa < 2:97775/140‑1 (MQ=255) aGTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTaaa > 2:335971/1‑140 (MQ=255) gTACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAg < 1:392586/140‑1 (MQ=255) tACAGAATACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAgg > 1:431307/1‑140 (MQ=255) aaTACTGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGtt > 1:384275/1‑140 (MQ=255) tGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAAttt > 1:498206/1‑140 (MQ=255) tGATATCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATATCTTACGTTAGGTCCTAGGCGTAAAGGACGTTAAttt > 1:239749/1‑140 (MQ=255) tCTGGCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTaa > 1:657008/1‑140 (MQ=255) ggCATATCTGCCC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAAACg > 2:595405/1‑140 (MQ=255) | GATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑CC‑CCC‑CCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAAACG > NC_000913/1818416‑1818672 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |