Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,842,809	(T)5→6	coding (439/1344 nt)	gdhA →	glutamate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,842,804	1	.	T	100.0%	72.4 / NA	23	R145L (CGT→CTT)	gdhA	glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (9/14);  total (9/14)

TTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCG‑TTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCA  >  NC_000913/1842679‑1842932                                                                                                                               |                                                                                                                                 ttCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGgcg                                                                                                                     <  2:601746/140‑1 (MQ=255)   ccATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCt                                                                                                                   <  2:651944/140‑1 (MQ=255)   ccATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCt                                                                                                                   <  1:656878/140‑1 (MQ=255)        ctcAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCtgatga                                                                                                              <  2:440373/140‑1 (MQ=255)                 cTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATTACTGAACTGt                                                                                                     >  2:438150/1‑140 (MQ=255)                   cGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTAt                                                                                                   >  2:13206/1‑140 (MQ=255)                                aCCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCGGCCACTGATGACCGAACACTATCGCCACCTGGgcg                                                                                      >  2:307144/1‑140 (MQ=255)                                aCCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGgcg                                                                                      <  2:383768/140‑1 (MQ=255)                                                 gACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCg                                                                     <  2:113211/140‑1 (MQ=255)                                                  actactCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCgg                                                                    <  1:648355/140‑1 (MQ=255)                                                         tGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGa                                                             >  2:654448/1‑140 (MQ=255)                                                                                ggcAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAg                                      >  1:56101/1‑140 (MQ=255)                                                                                 gcAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATTACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGt                                     <  1:438150/140‑1 (MQ=255)                                                                                 gcAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGt                                     <  2:394536/140‑1 (MQ=255)                                                                                                 gAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCgggg                     >  1:482695/1‑140 (MQ=255)                                                                                                  aaaGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGga                    <  1:93528/140‑1 (MQ=255)                                                                                                  aaaGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGga                    >  2:63828/1‑140 (MQ=255)                                                                                                        aaaaGCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAtgatga              <  2:571217/140‑1 (MQ=255)                                                                                                            gCGAAGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGCTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGTTGATGgaaaa          >  2:652837/1‑140 (MQ=255)                                                                                                              gAAGGTGAAGTGATGCGTTTTTTGCCAGGCGTTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGc        <  2:274513/140‑1 (MQ=255)                                                                                                                aGGTGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGATGaaaaaaaat      >  2:95637/1‑136 (MQ=255)                                                                                                                   tGAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTcc   <  1:13206/140‑1 (MQ=255)                                                                                                                    gAAGTGATGCGTTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCca  <  2:594621/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                 TTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAAAATGCCCTGACTACTCTGCCGATGGGCGGTGGTAAAGGCGGCAGCGATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCG‑TTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCA  >  NC_000913/1842679‑1842932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑