| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,855,756 | (T)7→8 | coding (216/981 nt) | ydjG ← | NADH‑dependent methylglyoxal reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,855,749 | 1 | . | T | 90.2% | 124.3 / 6.1 | 41 | coding (223/981 nt) | ydjG | NADH‑dependent methylglyoxal reductase |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base T (14/23); total (16/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑G‑TTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGC > NC_000913/1855618‑1855880 | tACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑G‑ttttt‑ttt < 1:68174/140‑2 (MQ=255) tCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCt > 2:162744/1‑140 (MQ=255) tCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCt > 1:68041/1‑140 (MQ=255) gcgGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACACCCTGTTCACGGGGCACG‑TTTTT‑TTAACGCCTGa > 2:291725/1‑140 (MQ=255) cgGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGAc > 1:620081/1‑140 (MQ=255) tAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACcgac > 2:592466/1‑140 (MQ=255) tAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACcgac < 1:87020/140‑1 (MQ=255) aGATTCCGGGGAAAGGTTTTTATACAACTGCTGATGGCCTACTTTGTTTAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACcgacg < 2:217316/140‑1 (MQ=255) aGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACcgacg < 1:162744/140‑1 (MQ=255) aTTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGAt < 2:446094/140‑1 (MQ=255) aTTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCATTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGAt < 2:611142/140‑1 (MQ=255) ttCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATa < 2:527124/140‑1 (MQ=255) gggAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACCGGGCA‑G‑TTTTTGTTAACGCCTTACCGACGATAACTTc > 1:217316/1‑140 (MQ=255) gAAAGGTTTTTATACAGGTGCCGATCGCCAATTTTCTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCAc < 2:301243/140‑1 (MQ=255) aaaGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACt < 1:592466/140‑1 (MQ=255) tttATCCAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCa < 2:140446/140‑1 (MQ=255) tttATACAACTGCCGATCGCCATCTTTGTTGAAAAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCa < 2:301218/140‑1 (MQ=255) tttATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCa < 2:620081/140‑1 (MQ=255) aaCTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTtat > 1:405543/1‑140 (MQ=255) cTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTatat > 2:455569/1‑140 (MQ=255) ccGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCt > 2:77852/1‑140 (MQ=255) ccGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCt > 2:510005/1‑140 (MQ=255) ccGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCt > 1:463697/1‑140 (MQ=255) ccGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCATGAACGATGATAACTTCACTATTGCCAAAGTTATATCCt > 2:226903/1‑140 (MQ=255) tCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGgcg < 1:508683/140‑1 (MQ=255) tCGCCAACTTTGTTGAAGAAACTTCCTTTTCCTTCCCAGACAATGCCGAATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGgcg < 2:217410/140‑1 (MQ=255) tgttgAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTatcaat < 2:22378/140‑1 (MQ=255) ttgAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCGAAAGTTGTATCCTTGCGCAGTatcaatca > 1:210552/1‑140 (MQ=255) cTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCcaca > 2:145119/1‑140 (MQ=255) cTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCcaca > 2:452142/1‑140 (MQ=255) tCGTTCCCAGACAATGCCGCATTTGGTTTCTACTTCAACCTGTTCCCGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCTCTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGa < 1:623012/140‑1 (MQ=255) ttCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACATCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATgg > 2:387459/1‑140 (MQ=255) cccAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGc < 2:464645/140‑1 (MQ=255) tGCCGCATTTGGTTTCTACTACCACCTGTTCACGGTGCATT‑TTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAAt < 2:210552/140‑1 (MQ=255) gCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTa < 2:463697/140‑1 (MQ=255) cATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCTTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTAt < 1:510005/140‑1 (MQ=255) ctacAACCTGTTCACGGGGCAGG‑TTATT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTg < 1:226903/140‑1 (MQ=255) ctacAACCTGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTg < 1:11009/140‑1 (MQ=255) tGTTCACGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCga < 1:455569/140‑1 (MQ=255) aCGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCg < 1:499028/140‑1 (MQ=255) aCGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCg < 1:77852/140‑1 (MQ=255) cGGGGCA‑GTTTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGc < 1:145119/140‑1 (MQ=255) | TACCTCTTCGCGGATAGATTCCGGGGAAAGGTTTTTATACAACTGCCGATCGCCAACTTTGTTGAATAAACTTCCTTTTCGTTCCCAGACAATGCCGCATTTGGTTTCTACTACAACCTGTTCACGGGGCA‑G‑TTTTT‑TTAACGCCTGACCGACGATAACTTCACTATTGCCAAAGTTATATCCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGC > NC_000913/1855618‑1855880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |