| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 272,712 | (G)5→6 | intergenic (+58/‑135) | eyeA → / → ykfC | CP4‑6 prophage; small RNA EyeA/CP4‑6 prophage; PF00078 domain‑containing protein YkfC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 272,707 | 1 | . | G | 95.0% | 135.6 / 0.1 | 40 | intergenic (+53/‑140) | eyeA/ykfC | CP4‑6 prophage; small RNA EyeA/CP4‑6 prophage; PF00078 domain‑containing protein YkfC |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (18/20); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAA‑GG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAA > NC_000913/272571‑272841 | gcagcaGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAA‑gg‑g < 1:661983/140‑1 (MQ=255) agcagcGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAA‑gg‑g‑gg > 1:44510/1‑140 (MQ=255) gcGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAA‑gg‑g‑gggcat > 2:643860/1‑136 (MQ=255) gcGTCTATCACGCTTGCGTTGCAGACTTCTGCCAAGTTGAGGCAAGCATGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAA‑gg‑g‑gggcat < 2:262869/140‑5 (MQ=255) gCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGAcgcg < 2:42283/140‑1 (MQ=255) tGCTGACTTCTGCCAACTTGCGACAAGCAAGCATATAGACTGCGACGGACAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCgctgg < 2:241418/140‑1 (MQ=255) cTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCgctggct > 2:428131/1‑140 (MQ=255) ttCTGCCAACTTGCGGCAAGCAAGGATAAATAGTGTGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCgg < 2:192985/140‑1 (MQ=255) tCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGa < 1:109503/140‑1 (MQ=255) tGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc < 1:643860/140‑1 (MQ=255) tGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc > 2:79247/1‑140 (MQ=255) tGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCCGGCTGGCGGAGc > 2:347461/1‑140 (MQ=255) tGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCCGGCTGGCGGAGc < 1:347461/140‑1 (MQ=255) ccAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCtt > 2:591001/1‑140 (MQ=255) aaaGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa < 1:591001/140‑1 (MQ=255) tgcgagggCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCa < 1:453894/135‑1 (MQ=255) gCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCa > 1:591175/1‑140 (MQ=255) gCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAACGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCa > 1:439367/1‑140 (MQ=255) cGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCAt > 1:547179/1‑140 (MQ=255) cGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTAGCGAAGCGTCCGTAGTCGTCCTCGATGGGGAAAGCCCAt > 2:197741/1‑140 (MQ=255) cGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACCCGCTGGCTCGCGAAACATCCGTAGTCGTCCGCGATGGGGAAAGCCCAt > 2:323510/1‑140 (MQ=255) aCGGGCAGCCTCCTCTGTATGCCTGGGTCTAGGCAGGGAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTa < 1:323510/140‑1 (MQ=255) gCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGACTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCg < 1:398627/140‑1 (MQ=255) cctcctCAGTATGCCCGAGTCCCGGTAGGGAAACCGGCGAAGGTCAGCGACGGA‑GTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGaa < 1:201751/140‑1 (MQ=255) aGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg < 2:563535/140‑1 (MQ=255) aGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg < 2:165061/140‑1 (MQ=255) ccTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGttt > 1:106006/1‑140 (MQ=255) cTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg < 2:661623/133‑1 (MQ=255) cTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg > 1:661623/1‑133 (MQ=255) cTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGAGAAAAGCCCATTACATGGTGAAGGGAGGCAGTTTg > 2:319207/1‑140 (MQ=255) tCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAAtgt > 1:655361/1‑140 (MQ=255) ggcaggTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGttt > 1:377632/1‑140 (MQ=255) tAAACCGGGGAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACg > 2:151670/1‑140 (MQ=255) ggAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTaa < 2:148218/140‑1 (MQ=255) ggAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTaa < 1:79247/140‑1 (MQ=255) gAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTg > 1:79648/1‑126 (MQ=255) gAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTg < 2:79648/126‑1 (MQ=255) gAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAAc > 1:26585/1‑140 (MQ=255) gAAGGTCAGCGACGGATGTTAAGGG‑G‑GGCATGGCCCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCAAAGTGAAACAGTTTGATTGTGATTGCGACGTGAATTAAc > 2:286527/1‑140 (MQ=255) cGACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAAc < 2:411424/140‑1 (MQ=255) cGACGGATGTTAA‑GGTG‑GGCATTGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAAc < 1:260076/140‑1 (MQ=255) gACGGATGTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACg < 2:439367/140‑1 (MQ=255) gACGGATGTTAA‑GG‑GAGGCATGGCTGCGACGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACg < 1:319207/140‑1 (MQ=255) gTTAAGGG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGaa < 2:547179/140‑1 (MQ=255) | GCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAA‑GG‑G‑GGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAA > NC_000913/272571‑272841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |