| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,734,543 | (C)8→10 | coding (492/732 nt) | pgeF ← | polyphenol oxidase YfiH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,734,535 | 1 | . | C | 100.0% | 52.6 / NA | 16 | E167G (GAG→GGG) | pgeF | polyphenol oxidase YfiH |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (10/6); total (10/6) | |||||||||||
| * | NC_000913 | 2,734,535 | 2 | . | C | 93.8% | 49.4 / ‑2.8 | 16 | coding (500/732 nt) | pgeF | polyphenol oxidase YfiH |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (9/6); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC > NC_000913/2734407‑2734662 || tttGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCT‑‑cccccccccca < 2:342363/140‑4 (MQ=255) tGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCT‑‑ccccccccccact < 1:13875/140‑6 (MQ=255) cGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGAcc > 2:147224/1‑140 (MQ=255) gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTg < 1:115367/140‑1 (MQ=255) gCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTGCTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTAGAATCCGCGTCGGCCAATTGCCGGCCCTAACCCgg > 2:250473/1‑140 (MQ=255) gCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAgg < 1:374249/140‑1 (MQ=255) tCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACCTCGAACGCTCGTCGACCAATTGCCGTCCCTAACCAGGCGAGAATAttt > 2:224031/1‑140 (MQ=255) cAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACCCCC‑‑CCCCCCCCCCCCCCAAGCCGCGCGGGCCAATTGCGGCCGCTAACAGGGAGGAAATATTTTCCgg > 2:369808/1‑140 (MQ=255) aTACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGAtt > 1:640699/1‑140 (MQ=255) tCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa < 1:147224/140‑1 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTTTCAGCaaaaa > 1:457914/1‑139 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc > 1:53409/1‑140 (MQ=255) tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg > 2:455949/1‑140 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa > 1:575953/1‑140 (MQ=255) gaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa < 1:138335/140‑1 (MQ=255) aaatgaaACGTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa < 2:655276/139‑1 (MQ=255) acaggCGTCTACTGCCCTAAACTCCTCGCGAGCT‑‑CCC‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGcc < 2:295621/136‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:30150/140‑1 (MQ=255) cATAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTACCTGCACGCGTGCGCACAGTcctgt > 1:295621/1‑137 (MQ=255) aTAAACGCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgc < 1:27202/140‑1 (MQ=255) gCCTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgccagcca > 1:62288/1‑140 (MQ=255) cTCGCGA‑‑A‑‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCcagccagc > 1:334819/1‑140 (MQ=255) || TTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑A‑‑CCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC > NC_000913/2734407‑2734662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |