| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,949,993 | (T)5→6 | coding (752/1332 nt) | argA → | N‑acetylglutamate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,949,988 | 1 | . | T | 97.0% | 106.6 / ‑3.3 | 33 | coding (747/1332 nt) | argA | N‑acetylglutamate synthase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (15/17); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑TTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAA > NC_000913/2949858‑2950121 | ttGGTTTTTGCTCTTCCCAGGGTGTCACTAATGACAACGTTGATATTGCCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑tttt‑ttgcg < 2:235957/140‑5 (MQ=255)ttGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑tttt‑ttgcg < 2:561153/140‑5 (MQ=255)ttGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑tttt‑ttgcg < 2:46356/140‑5 (MQ=255) cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc > 2:630076/1‑140 (MQ=255) cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc > 1:147627/1‑140 (MQ=255) cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc > 2:11442/1‑140 (MQ=255) cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc > 1:426148/1‑140 (MQ=255) cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc > 1:459876/1‑140 (MQ=255) cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑TTTTATGCGTGACGCAGTTAAAGCCTGCCGCAgc > 1:328559/1‑140 (MQ=255) aCTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgcg > 1:36392/1‑140 (MQ=255) aTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgt < 1:630076/140‑1 (MQ=255) acgacgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcg < 1:126981/140‑1 (MQ=255) acgacgGTGATATTGTCTCCAAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcg < 1:146344/140‑1 (MQ=255) acgGTGATATTGTCTTCGAACTCTTTCCTCACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg < 1:228174/140‑1 (MQ=255) tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt > 1:466993/1‑140 (MQ=255) tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt > 2:663441/1‑140 (MQ=255) tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt < 1:663441/140‑1 (MQ=255) tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCACAGAGGCGTGCGTAGCTGTCATTTAATCa > 1:230644/1‑140 (MQ=255) tCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGa > 2:476335/1‑140 (MQ=255) gAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGCGAACGGCTATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcg < 2:230644/140‑1 (MQ=255) aagcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgc < 1:572820/140‑1 (MQ=255) aagcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgc < 2:142064/140‑1 (MQ=255) agcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcg < 2:69037/140‑1 (MQ=255) gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc < 2:459351/140‑1 (MQ=255) gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc < 1:476335/140‑1 (MQ=255) caagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGt < 2:147627/140‑1 (MQ=255) agagAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGTAGATGGCCCGCTGTTGCAAGAGTTGTTCTCACGCGAc > 1:296338/1‑140 (MQ=255) ggCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCg > 1:506134/1‑140 (MQ=255) gCGATTACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCgg < 2:421672/140‑1 (MQ=255) ttACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg < 2:36392/140‑1 (MQ=255) ttACAACTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCGGTTGCAAAAGTGGTTCTCACGCGACGGTATCGGTACg > 1:323942/1‑140 (MQ=255) aaCTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa < 2:506134/140‑1 (MQ=255) aaCTCCGGTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa < 1:550547/140‑1 (MQ=255) aaCTCCGGCACGGTGCGTT‑TTTT‑TGCGTGGTTCCGCGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa < 2:328559/140‑1 (MQ=255) gTACGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGGTATCAGGAAGATGGCGCGCAGTTGCAAGTGTTATTCGCACGCGACAGTATAGGTACGCAGATTGTGAt > 2:235171/1‑140 (MQ=255) aCGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGGTGCAAGAGGTGTACTCTCGCGACGGTATCGTTACGCAGATTGTGATgg > 2:175059/1‑140 (MQ=255) cGGTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGa > 2:545916/1‑140 (MQ=255) gTGCG‑CTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGaaa < 1:11442/140‑1 (MQ=255) | TTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑TTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAA > NC_000913/2949858‑2950121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |