| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,999,680 | (G)7→9 | coding (212/756 nt) | ygeR ← | LysM domain‑containing putative peptidase lipoprotein YgeR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,999,673 | 1 | . | G | 100.0% | 93.9 / NA | 28 | P73P (CCT→CCC) | ygeR | LysM domain‑containing putative peptidase lipoprotein YgeR |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (14/14); total (14/14) | |||||||||||
| * | NC_000913 | 2,999,673 | 2 | . | G | 96.4% | 93.3 / ‑2.9 | 28 | coding (219/756 nt) | ygeR | LysM domain‑containing putative peptidase lipoprotein YgeR |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (13/14); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01 | |||||||||||
GCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGT > NC_000913/2999534‑2999792 || gcggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCGCCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTaa < 2:13784/140‑1 (MQ=255) cAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGAACAACTTCAATGGTGTAA‑‑‑ggggg > 1:159703/1‑140 (MQ=255) gatgatTTCGGTACCGCTGATGTCGGTGTAAACGATGCGTTTTTGGTCCTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTG‑AA‑‑‑ggggggggg < 2:179669/140‑3 (MQ=255) gatTTCGGTACCGCTTATGACGTTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑‑gggggggggaa < 1:271268/140‑5 (MQ=255) atTTCGGTTCCGCTGAATACGGAGTAACCGATGAGATTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑‑gggggggggaaa < 2:307434/140‑6 (MQ=255) cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTc > 2:472330/1‑140 (MQ=255) gaCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGCCCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGcc < 1:79548/140‑1 (MQ=255) aCCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCtttt < 2:159703/140‑1 (MQ=255) gATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTGCTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACg < 1:306964/140‑1 (MQ=255) aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGc > 2:647146/1‑140 (MQ=255) gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGGGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTg > 2:535858/1‑140 (MQ=255) aTTTGGCGTTTGATTTTAGTGTTATACTGCTCCATTTCGCCCCACCCACTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 2:319878/140‑1 (MQ=255) aTTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 1:85469/140‑1 (MQ=255) aTTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 1:507604/140‑1 (MQ=255) gCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa < 2:377907/140‑1 (MQ=255) aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcccc > 1:544838/1‑140 (MQ=255) aTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATccccc > 2:354346/1‑140 (MQ=255) aTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCGTTTACGCTGGTTTCCGGGGTGCGCGGAATACGATATAGCGTATccccc > 2:169429/1‑140 (MQ=255) aCTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc > 1:77479/1‑140 (MQ=255) aCTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc < 2:43583/140‑1 (MQ=255) aCTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc < 1:515042/140‑1 (MQ=255) aCTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc < 1:468139/140‑1 (MQ=255) cTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGTTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc > 1:260017/1‑140 (MQ=255) ttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGttt < 1:572543/133‑1 (MQ=255) ttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGttt > 2:572543/1‑133 (MQ=255) ttCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGgtg > 2:30758/1‑140 (MQ=255) ttttAGTTTCTGACCAACTTCAATGGTGTAAG‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGaa > 2:586262/1‑140 (MQ=255) tAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 1:472330/140‑1 (MQ=255) tAGTTTCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 1:354346/140‑1 (MQ=255) tCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTc > 1:625145/1‑140 (MQ=255) tCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTc > 1:340125/1‑140 (MQ=255) tCTGACCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTc < 1:647146/140‑1 (MQ=255) aCCAACTTCAATGGTGTAAGG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGATCCCGTGGTGCGCGAAATACGATATATCGTATCCCTCCGTTTCTCGGTGTAACCGGAGGCGGAATACGTTCCTGt > 2:296115/1‑140 (MQ=255) || GCGGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGT > NC_000913/2999534‑2999792 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |