| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,001,304 | (G)7→8 | coding (960/2259 nt) | xdhA → | putative xanthine dehydrogenase molybdenum‑binding subunit XdhA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,001,297 | 1 | . | G | 96.8% | 101.2 / NA | 32 | A318G (GCG→GGG) | xdhA | putative xanthine dehydrogenase molybdenum‑binding subunit XdhA |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base G (18/12); minor base A (1/0); total (19/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑GGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTT > NC_000913/3001159‑3001427 | gcgtgctccGTGCCCCCCGCGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCGGTCTAACACCGGCGGTTATGCATCTCGCGGGCGCTCCATCGCTTCTGC‑g < 2:104622/133‑1 (MQ=255) cAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑gggg > 2:157845/1‑140 (MQ=255) aCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTAATGCATCCCACGGGCACTCCATCGCTTCTGC‑gggg‑gg > 1:304343/1‑140 (MQ=255) ccACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTGATGCATCTTACGAGCAGTCCATCGCTTCTGCAGGGG‑GGGGATAAAGTc > 2:321742/1‑140 (MQ=255) cGCTTTTACCATTGACCGGCAACTGGGCGTGTACCGCGAGGGAACATTCAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCt < 2:316187/140‑1 (MQ=255) cTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTa < 2:373181/140‑1 (MQ=255) ttACCATTGACGGGCTAATGGGAGTGAACAGCGACGGTACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCt < 1:313917/140‑1 (MQ=255) gggCGTGAACCGCGACCAAACATTGAAAGGTCATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTAc < 2:304343/140‑1 (MQ=255) gAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTAc < 2:17974/140‑1 (MQ=255) aaCATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGAcc < 1:636183/140‑1 (MQ=255) tataGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAcc > 2:359514/1‑140 (MQ=255) tataGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAcc > 2:146290/1‑140 (MQ=255) ataGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCt > 2:514121/1‑140 (MQ=255) taGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTc > 2:139832/1‑140 (MQ=255) taGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGACTACGCTAACAATTAAAAGACTTGCTATACCAACCTc > 1:238695/1‑140 (MQ=255) gTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTccc > 2:17620/1‑140 (MQ=255) ggATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCg > 1:21739/1‑140 (MQ=255) ggATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTCCCTATGCTCACATTTCAAAGATCTGCGATACCAACCTCCCCTCg > 2:280330/1‑140 (MQ=255) gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTGGTGCCTACACTTGCAGGTCAAAGACCTGCTATACCAACCTCCCCTCgg > 2:206358/1‑140 (MQ=255) tGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCt < 1:37635/140‑1 (MQ=255) cTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGATCTGGTAGACCAACGTCCCCTCGGCTGGTg > 1:182871/1‑140 (MQ=255) cTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTg < 1:359514/140‑1 (MQ=255) gTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCg < 2:88552/140‑1 (MQ=255) gTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCg < 1:466092/140‑1 (MQ=255) tAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATg > 2:118289/1‑140 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTa > 1:482179/1‑140 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTAAACCAACCTCCCCGCGGCTAGTGCGATGCGTGGTTa > 2:309145/1‑140 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCAATACCAAACTCACCCCGGCTGGTGCGATGCGTGGTTa > 1:227306/1‑140 (MQ=255) ttATGCATCTCACGGTCACTTCTTCTCTTGTGC‑GGGGCGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGgc < 1:205055/140‑1 (MQ=255) aTCTCACGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCca < 2:58031/140‑1 (MQ=255) cGGGCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGATTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTc > 1:475775/1‑140 (MQ=255) gggCACTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCg > 2:602768/1‑140 (MQ=255) cTCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTAttt > 2:397082/1‑140 (MQ=255) tCCATCGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATCCGTGGTTATGGCGTGCCACAAGTCGTATTTg > 2:206545/1‑140 (MQ=255) cGCTTCTGCGGGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt < 2:21739/140‑1 (MQ=255) | TCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑GGGG‑GGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTT > NC_000913/3001159‑3001427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |