Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,094	(G)8→9	noncoding (72/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,086	1	.	G	91.7%	111.7 / 3.5	36	noncoding (80/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (15/18);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGG  >  NC_000913/4605950‑4606215                                                                                                                                          |                                                                                                                                       caAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGa                                                                                                                                         >  2:370943/1‑137 (MQ=255) caAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGa                                                                                                                                         <  1:370943/137‑1 (MQ=255)    aCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGACAGGAAGTAAAGATAACTGGTGCGA‑‑c‑gg‑gg‑g                                                                                                                              <  2:412014/140‑1 (MQ=255)      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑g‑gg‑gg‑gg‑g                                                                                                                           >  1:401851/1‑140 (MQ=255)      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATAAAGGAAGACACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑g‑gg‑gg‑gg‑g                                                                                                                           >  2:241961/1‑140 (MQ=255)        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑g‑gg‑gg‑gg‑gga                                                                                                                         >  2:458623/1‑138 (MQ=255)        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑g‑gg‑gg‑gg‑gga                                                                                                                         >  1:84280/1‑138 (MQ=255)          gCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGCAAGAAACAGGAACGGAAGTAAAGATAATTGGTGCGA‑‑G‑GGCGG‑GG‑GACt                                                                                                                        >  2:299682/1‑140 (MQ=255)                  aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAAcccc                                                                                                                <  2:479301/140‑1 (MQ=255)                    aTTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCa                                                                                                              <  2:84280/140‑1 (MQ=255)                        tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTc                                                                                                          >  1:146156/1‑140 (MQ=255)                          cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCg                                                                                                        >  1:126856/1‑140 (MQ=255)                           tACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGt                                                                                                       >  1:141013/1‑140 (MQ=255)                             ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGG‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTa                                                                                                      <  1:77031/140‑1 (MQ=255)                             ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTa                                                                                                      >  1:139453/1‑139 (MQ=255)                             ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTa                                                                                                      <  2:139453/139‑1 (MQ=255)                              cATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAg                                                                                                    >  1:151204/1‑140 (MQ=255)                                  gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGaca                                                                                                <  2:612676/140‑1 (MQ=255)                                       tGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGt                                                                                                       <  1:375902/128‑1 (MQ=255)                                       tGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGt                                                                                                       >  2:375902/1‑128 (MQ=255)                                       tGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGt                                                                                                       <  1:160478/128‑1 (MQ=255)                                       tGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGt                                                                                                       >  2:160478/1‑128 (MQ=255)                                                   cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                               <  2:146156/140‑1 (MQ=255)                                                           atGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                       >  1:468283/1‑140 (MQ=255)                                                           atGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                       >  2:281467/1‑140 (MQ=255)                                                           atGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTGGAACCCCCACGTCCGTAAGGACACTCACACCTGAAGCTAGCGCGTCTa                                                                       >  2:241686/1‑140 (MQ=255)                                                                 atgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCGCCACGTCCGTAATGACGCTAACACCTGAAGTTAGCGCGTCTACCAAtt                                                                 >  2:192262/1‑140 (MQ=255)                                                                    atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                              <  2:141013/140‑1 (MQ=255)                                                                        tGAATTTGAATCAGGGATGAAAAATCAAGTAAGAAACTAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                          <  1:299682/140‑1 (MQ=255)                                                                           aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                       >  2:573642/1‑140 (MQ=255)                                                                             ttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                     <  1:573642/140‑1 (MQ=255)                                                                               gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                   <  2:151204/140‑1 (MQ=255)                                                                               gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                   <  1:82301/140‑1 (MQ=255)                                                                                aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                  <  1:584970/140‑1 (MQ=255)                                                                                aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                  <  2:401851/140‑1 (MQ=255)                                                                                          gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTa                                        <  1:458623/140‑1 (MQ=255)                                                                                                      aagaaACAAGAAAGGCAGTAAAAATTATTAGTGCGA‑‑GCGG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGata                            <  1:241961/140‑1 (MQ=18)                                                                                                      aagaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGata                            <  1:371157/140‑1 (MQ=255)                                                                                                                              tAATTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg    >  1:80017/1‑140 (MQ=255)                                                                                                                              aaaTTGGTGGGA‑‑G‑GG‑GG‑GGTGACTTGCACCCCGCCGGCCGTAAATGCACTAAATCCTGAAGATAGTGCGTCTACCAAATCCGCCATCTTCGAACAATGATCTAACAATTTTTGAAATCACCTCGTTTGGTGCGAgggggg    <  2:122385/139‑1 (MQ=255)                                                                                                                               aaTTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACt                                                                                              <  2:363108/49‑1 (MQ=11)                                                                                                                               aaTTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACt                                                                                              >  1:363108/1‑49 (MQ=11)                                                                                                                               aaTTGGTGCGAG‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTCGATCTCCCCTCGTTTGGTGCGAggggggg   >  1:240630/1‑140 (MQ=18)                                                                                                                                aTTGGTGCGA‑‑G‑GG‑GGTGG‑GACTAGACCCCCCACGTCCCTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggg  <  1:192262/140‑1 (MQ=21)                                                                                                                                          |                                                                                                                                       CAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑G‑GG‑GG‑GG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGG  >  NC_000913/4605950‑4606215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑