| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 685,941 | (C)6→7 | coding (729/741 nt) | gltJ ← | glutamate/aspartate ABC transporter membrane subunit GltJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 685,935 | 1 | . | C | 86.4% | 60.8 / 3.8 | 22 | coding (735/741 nt) | gltJ | glutamate/aspartate ABC transporter membrane subunit GltJ |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (5/14); total (7/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑C‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAA > NC_000913/685820‑686061 | caaaaaaTTCAAAGCACAACCGTCGTGACGGCGATTTGCCGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCg < 2:223769/137‑1 (MQ=255) aaTACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACtt < 2:376012/140‑1 (MQ=255) ccAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACg < 2:445740/140‑1 (MQ=255) cAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTAGTTG‑C‑CCCGC‑CAGGTGGCCAGGAAGGCGAACTTTACGt > 1:200177/1‑140 (MQ=255) tCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCa > 1:360063/1‑140 (MQ=255) tCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATATG‑CTCCC‑T‑CATGTTGCCAGGCAGGCGAACTTTACGTTCa > 2:196291/1‑140 (MQ=255) cctcccgccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAAc < 2:444822/136‑1 (MQ=255) tACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCa < 1:349292/140‑1 (MQ=255) tACCGCCGTGACGGTCATTTTCCGGATGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCa < 1:196291/140‑1 (MQ=255) ccgccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑C‑CCC‑CACATGTTGGCAGGCAGGCGAACGTTACGTTCAACCAGc > 1:172630/1‑140 (MQ=255) gACGGTGATTTTCAGAGTGATCGCCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGtcatca < 1:280994/140‑1 (MQ=255) aCGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCAc < 1:534585/140‑1 (MQ=255) gTGATTTTCAGGGTGATCACCATCCCGTCGACCAGCTATCGCAGGGAATGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAg < 2:172630/140‑1 (MQ=255) tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATa < 2:546380/140‑1 (MQ=255) aGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCg < 2:462544/123‑1 (MQ=255) aGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCg > 1:462544/1‑123 (MQ=255) atGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTAATTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTa < 2:525806/140‑1 (MQ=255) tGGAACTCCAGTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTcc > 2:354511/1‑140 (MQ=255) gTCAAACTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACCCCCAGGCATggg > 2:421357/1‑140 (MQ=255) gTCAAACTCGTACATTAATTG‑CGCCC‑C‑CATGGTGCGAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATggg < 2:200177/140‑1 (MQ=255) aaaCTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAAAGACTCCCCGGCATGGGGTg > 1:492477/1‑140 (MQ=255) aCTCGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAg < 2:395634/140‑1 (MQ=255) cGTACATTATTTGCC‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTaa < 2:453290/140‑1 (MQ=255) | CACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑C‑CCC‑C‑CATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAA > NC_000913/685820‑686061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |