| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 717,125 | (G)6→8 | coding (1141/1320 nt) | potE ← | putrescine:H(+) symporter/putrescine:ornithine antiporter PotE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 717,119 | 1 | . | G | 89.4% | 143.7 / 10.6 | 47 | coding (1147/1320 nt) | potE | putrescine:H(+) symporter/putrescine:ornithine antiporter PotE |
| Reads supporting (aligned to +/- strand): ref base . (4/1); new base G (20/22); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
| * | NC_000913 | 717,119 | 2 | . | G | 89.1% | 141.3 / 10.6 | 47 | coding (1147/1320 nt) | potE | putrescine:H(+) symporter/putrescine:ornithine antiporter PotE |
| Reads supporting (aligned to +/- strand): ref base . (4/1); new base G (19/22); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGA‑‑G‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAGCGACGGGCTAATGG > NC_000913/716980‑717247 || gATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGa < 1:565814/140‑1 (MQ=255) aGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGGTTGCAACTTGCGCTTTTGA‑‑g‑‑g‑‑gg‑‑g‑‑g > 1:233151/1‑140 (MQ=255) aCCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCCCGCCAACAAAAGGAACGAAGTTTGCGACTTACGCTTTTGA‑‑g‑‑g‑‑gg‑‑g‑‑ggg > 2:191184/1‑138 (MQ=255) ccATACAGTGTCCAACCTAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGA‑‑g‑‑g‑‑gg‑‑g‑‑gg < 2:39448/138‑2 (MQ=255) ccATACAGTGTCCAACCTAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGA‑‑g‑‑g‑‑gg‑‑g‑‑gg > 1:39448/1‑137 (MQ=255) aCAGTGTCCAACCAAGCAAGGTCACGATGGCACCGAACAGCATGGCTTTTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGA‑‑g‑‑g‑‑gg‑‑g‑‑gggcacat < 2:324837/140‑8 (MQ=255) tgtCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGc < 2:467131/140‑1 (MQ=255) gtCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGA‑‑G‑‑G‑‑GG‑‑G‑‑GCACATTAGCGAc > 2:461871/1‑140 (MQ=255) gtCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGGTTGCAACGTTCGCTATTGA‑‑GGTG‑‑GG‑‑G‑‑GCACATTAGCg > 1:209445/1‑140 (MQ=255) tCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGa > 1:610749/1‑140 (MQ=255) tCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGa > 1:573879/1‑140 (MQ=255) tCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGa > 2:577156/1‑140 (MQ=255) tCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTCTGCAACTGTCGCTTATGA‑‑G‑‑GGAGG‑‑G‑‑GCACATTAGCGa > 1:292123/1‑140 (MQ=255) ccaaccaaGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTTGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGAc < 2:362621/140‑1 (MQ=255) ccaaccaaGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCg < 2:61300/138‑1 (MQ=255) ccaaccaaGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCg > 1:61300/1‑138 (MQ=255) ccaaccaaGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGAc > 2:458807/1‑140 (MQ=255) ccaaccaaGGAAGGTCACGATGCAACCGTACAGCACGGCTTCTTCCCCCGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGAc < 2:184030/140‑1 (MQ=255) ccaaccaaGGAAGGTCACGATGAAAGCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGAc < 1:233897/140‑1 (MQ=255) aaccaaGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCt > 1:1862/1‑140 (MQ=255) aggaaggTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTGTCGCTTTTGAGTG‑‑G‑‑GG‑‑G‑‑GCACTTTAGCGACCTTCTGa > 1:224474/1‑140 (MQ=255) gaaggTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGaat > 1:445395/1‑140 (MQ=255) gaaggTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTAGCTTTTGA‑‑G‑‑G‑‑GG‑‑GGTGCACATAAGCGACCTTCTGaat > 1:326247/1‑140 (MQ=255) aaCCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATgcgg > 1:112215/1‑138 (MQ=255) aaCCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGA‑‑G‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATgccgcc < 1:461871/140‑1 (MQ=255) aCCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATgtcgc > 1:491247/1‑140 (MQ=255) gTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCAt > 2:661033/1‑140 (MQ=255) tACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCAAATTAGCGACCTACTAAATAATGACTAATGCCGCCAtt > 1:244702/1‑140 (MQ=255) aCAGCATGGCTTCTTCCCCGGATGAGTACAGGGCAAAAACGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTg < 2:233151/140‑1 (MQ=255) aGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGAc < 2:610749/140‑1 (MQ=255) aGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGAc < 1:96032/140‑1 (MQ=255) ggCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAat > 2:546595/1‑140 (MQ=255) cttcttCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAatat > 2:592920/1‑140 (MQ=255) ttcCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACgg < 1:345096/140‑1 (MQ=255) ccccGGATGAGTACATCGCATATAACCTATACATCGCCCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGaa < 1:191184/140‑1 (MQ=255) ccccGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGaa < 1:661033/140‑1 (MQ=255) cgcATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGAGAGAATAAACGGAAAGATATTGGTcaccac > 1:252930/1‑140 (MQ=255) cgcATAAAAGCTATACATCGCGCCAACAAAAACAACAAAGTTTGCAACTTTCCCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATCAGCGACCCTCTTAATAAAGACTAATGCCGCCATTGACAGAATATACGGAATCATATTGGTcaccac > 2:350175/1‑140 (MQ=255) cATAAAAGCTATACATCTCGCCCACAAAAGCAATACAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACgg < 2:326247/140‑1 (MQ=255) cATAAAAGCTATACATCGCGCCCACAAAAGCAACACAGTCTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACgg < 2:224474/140‑1 (MQ=255) cATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTCGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACgg < 2:432730/140‑1 (MQ=255) cTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAGTGATATTGGTcac > 1:571552/1‑127 (MQ=255) cTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTcac < 2:571552/127‑1 (MQ=255) cATCGCGCCAACAAAAGTAACAAAGTTTGCACCTTCCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAg < 2:278547/140‑1 (MQ=255) cgcgCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGA‑‑G‑‑G‑‑GGGTG‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCAc > 2:429965/1‑140 (MQ=255) cgcCAACAAAAGCAACAAAGTTCGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGt > 1:149109/1‑140 (MQ=255) caacaaAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGa < 1:41867/140‑1 (MQ=255) aaaGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGTCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAactga < 2:491247/140‑1 (MQ=255) aaaGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGTACGTTGAactga < 2:1862/140‑1 (MQ=255) aaaGCAACAAAGTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAactga < 1:592920/140‑1 (MQ=255) aaaGCAACAAAGTTTGCAACTTGTGATTTTGG‑‑G‑‑G‑‑GGGAG‑‑GCACATTAGCGACCTTCTGAATAATGGCTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAactga < 1:275851/140‑1 (MQ=255) gTTTGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTTACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAGCGa < 2:112215/140‑1 (MQ=255) tGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAactgactg < 2:552114/129‑1 (MQ=255) tGCAACTTTCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAactgactg > 1:552114/1‑129 (MQ=255) tCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAGCGACGGGCTAATgg > 2:512872/1‑140 (MQ=255) tCGCTTTTGAGGG‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAGCGACGGGCTAATgg > 2:130400/1‑140 (MQ=255) || GATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGA‑‑G‑‑G‑‑GG‑‑G‑‑GCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAGCGACGGGCTAATGG > NC_000913/716980‑717247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |