| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,104,948 | (T)8→9 | intergenic (+46/‑13) | csgA → / → csgC | curlin, major subunit/inhibitor of CsgA amyloid formation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,104,940 | 1 | . | T | 95.8% | 158.5 / ‑0.4 | 48 | intergenic (+38/‑21) | csgA/csgC | curlin, major subunit/inhibitor of CsgA amyloid formation |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (23/23); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
GGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGT > NC_000913/1104801‑1105071 | ggtggtGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTg > 2:483116/1‑140 (MQ=255) gCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑tttt‑ttt > 2:455950/1‑140 (MQ=255) gCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑tttt‑ttt > 2:374389/1‑140 (MQ=255) gCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑tttt‑ttt > 1:69645/1‑140 (MQ=255) ggTGCTGCAGTTGACCAAACTGTATCTAACTCCTCCGTCAACGTGACTTAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑tttt‑tttttcgg < 2:217535/140‑5 (MQ=255) tgctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGga < 1:376623/140‑1 (MQ=255) gctgcAGTTGACCAGGCTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGgag < 1:291383/140‑1 (MQ=255) gctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGgag < 1:119094/140‑1 (MQ=255) gctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGgag < 1:612155/140‑1 (MQ=255) gctgcAGTTGACCAGACCGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGgag > 2:555461/1‑140 (MQ=255) ttGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAata > 2:290646/1‑140 (MQ=255) gACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATg > 2:79570/1‑140 (MQ=255) gACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATg > 1:133099/1‑140 (MQ=255) ccAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGaa < 1:455950/140‑1 (MQ=255) aGACTGCATCTAACTCCTCCGTCAACGTTACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTT‑TTTTCGGGAGAAGAATATGAATAc < 1:466270/140‑1 (MQ=255) aGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATa < 2:111135/140‑1 (MQ=255) aGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTGGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATa < 1:256262/140‑1 (MQ=255) tcctccGTCAACGTGACTCAGGTTGGCTTTGGTAAAAACGCGACCGCTTATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCtt < 2:242364/140‑1 (MQ=255) ctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGATAAGGATGTGAATAGGTTATTACTCCTTGc > 1:225244/1‑140 (MQ=255) ctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGc > 1:604191/1‑140 (MQ=255) ccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg > 2:436758/1‑140 (MQ=255) aCGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGCAGAGGAATATGAATACGTTATGACTCCTTGCGGCACttt > 2:255423/1‑140 (MQ=255) tCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCa > 2:394090/1‑140 (MQ=255) ggTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGAt < 1:79570/140‑1 (MQ=255) cTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCtt < 1:290646/140‑1 (MQ=255) ggTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATCAATACGTTGTTAGTCCGTGCGGCACTTTCCAGTCAGATAACCTTTAAt > 2:206234/1‑140 (MQ=255) cgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGc < 1:483116/140‑1 (MQ=255) aCCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCaa > 2:76621/1‑140 (MQ=255) aCCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCaa > 1:511382/1‑140 (MQ=255) cTCATCAGTACTAATACTTCATTTGTATTACTGAATCAGGGCGCAAGCGCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg < 2:267826/140‑1 (MQ=255) cTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAgggg < 2:133099/140‑1 (MQ=255) tcatcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTGATTTCTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGa < 1:65517/140‑1 (MQ=255) catcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAt < 1:503625/140‑1 (MQ=255) cTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCCAGGGGATGTGTATAc > 1:266543/1‑140 (MQ=255) aTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTCTTACGACCAAGCATGGGGATGTGTATACCat > 2:323541/1‑140 (MQ=255) atcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCattatt > 1:46626/1‑140 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTGAGATAACCGTTACTACAACCCAGCATGGGGGTGTGTATACCATTATTc > 2:184469/1‑140 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc > 2:575305/1‑140 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc > 1:95029/1‑140 (MQ=255) tcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTc > 1:488122/1‑140 (MQ=255) tttGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTg > 1:394470/1‑140 (MQ=255) tGTATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGaa > 1:110676/1‑140 (MQ=255) tATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGt < 2:110676/140‑1 (MQ=255) tATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGt < 2:69645/140‑1 (MQ=255) tATTACAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGt < 2:139944/140‑1 (MQ=255) tATTACAGAAACAGGGCACAAGCCCTG‑TTTTCTTTGCGGCAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGt < 2:225244/140‑1 (MQ=255) aCAGAAACAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCAct < 2:626954/140‑1 (MQ=255) aCAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACt > 2:7064/1‑140 (MQ=255) cAGGGCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTCCTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTc < 2:151881/140‑1 (MQ=255) ggCGCAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAAt < 1:575305/140‑1 (MQ=255) gcgcAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATc > 1:656560/1‑140 (MQ=255) cgcAAGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCt < 2:604191/140‑1 (MQ=255) aaGCCCTGTTTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGt < 1:555461/140‑1 (MQ=255) | GGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑TTTT‑TTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGT > NC_000913/1104801‑1105071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |