Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,440,091	A→G	Y232H (TAC→CAC)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,440,091	0	A	G	100.0%	131.2 / NA	37	Y232H (TAC→CAC)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/19);  total (18/19)

CCAATGGCAGAGCCCATCAGGATAATCACCCGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGA  >  NC_000913/1439955‑1440224                                                                                                                                         |                                                                                                                                      ccAATGGCAGAGCCCATCAGGATAATCACCCGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCa                                                                                                                                    >  1:373216/1‑140 (MQ=255) ccAATGGCAGAGCCCATCAGGATAATCACCCGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGAACTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCa                                                                                                                                    <  2:588756/140‑1 (MQ=255)          gagCCCATCAGGATAATCACCCGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTtggt                                                                                                                           <  2:425338/140‑1 (MQ=255)                aTCAGGATAATCACCCGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTc                                                                                                                     >  1:596822/1‑140 (MQ=255)                 tCAGGATAATCACCCGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTcg                                                                                                                    >  1:75938/1‑140 (MQ=255)                       tAATCACCCGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGAc                                                                                                              >  1:643095/1‑140 (MQ=255)                          tCACCCGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTgg                                                                                                           >  1:39221/1‑140 (MQ=255)                             cccGTTCCGCTTGCGTATGCCCATACTATTCAAACGGTTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGaaa                                                                                                        <  1:285567/140‑1 (MQ=255)                              ccGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAAt                                                                                                       <  1:92647/140‑1 (MQ=255)                              ccGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCAAGGGTTGGTGGCTTCGCGAGACTGGAAAt                                                                                                       <  1:23428/140‑1 (MQ=255)                                  tCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAgt                                                                                                   >  1:398430/1‑140 (MQ=255)                                           ggATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGAtt                                                                                          <  2:373216/140‑1 (MQ=255)                                                     aaTATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTa                                                                                <  1:497855/140‑1 (MQ=255)                                                      atatTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTAc                                                                               <  2:398430/140‑1 (MQ=255)                                                        atTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACcg                                                                             <  2:161811/140‑1 (MQ=255)                                                                  cttaTACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCg                                                                   >  1:479066/4‑140 (MQ=255)                                                                          gacgacCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTcc                                                           >  2:488424/1‑140 (MQ=255)                                                                           acgacCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCgg                                                                  >  1:584366/1‑132 (MQ=255)                                                                           acgacCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCgg                                                                  <  2:584366/132‑1 (MQ=255)                                                                            cgacCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCgg                                                         >  2:656809/1‑140 (MQ=255)                                                                             gacCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCggg                                                        <  2:596822/140‑1 (MQ=255)                                                                               ccTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATGAGTGTCAGGATTGGCGTACGTACCGGGGATGACCGGATGTTCCGGGtt                                                      >  2:174873/1‑140 (MQ=255)                                                                                cTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                                     >  2:93300/1‑140 (MQ=255)                                                                                         cAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCgg                                            >  1:573023/1‑140 (MQ=255)                                                                                              aaaTCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGcgcg                                       <  1:656809/140‑1 (MQ=255)                                                                                              aaaTCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGcgcg                                       <  2:643095/140‑1 (MQ=255)                                                                                                   attcatCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAg                                  >  1:342766/1‑140 (MQ=255)                                                                                                      catCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCAt                               <  1:93300/140‑1 (MQ=255)                                                                                                        tCGCCTGTTCAACATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCa                             >  2:607873/1‑140 (MQ=255)                                                                                                                   aCATGGTCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTg                  <  1:488424/140‑1 (MQ=255)                                                                                                                         tCATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCAt            <  2:342766/140‑1 (MQ=255)                                                                                                                          cATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATg           <  2:11104/140‑1 (MQ=255)                                                                                                                          cATAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATg           <  2:75938/140‑1 (MQ=255)                                                                                                                            tAGACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATgag         <  2:479066/140‑1 (MQ=255)                                                                                                                              gACCGCGTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGAt       >  1:550458/1‑140 (MQ=255)                                                                                                                                   cgTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGa  >  2:472937/1‑140 (MQ=255)                                                                                                                                   cgTTGTGCCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCAGCAACTTCGACCTGAGGCATGAGATCAAGa  >  1:282364/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CCAATGGCAGAGCCCATCAGGATAATCACCCGTTCCGCTTGCGGATGCCCGTAATATTCAAACGGCTGATACTGACGACCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGACCTGCGGCATGAGATCAAGA  >  NC_000913/1439955‑1440224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑