| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,453,575 | T→C | H23R (CAC→CGC) | paaZ ← | oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,453,575 | 0 | T | C | 97.6% | 136.9 / ‑4.8 | 43 | H23R (CAC→CGC) | paaZ | oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (25/16); total (26/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CGCCGCACGTTCGATAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAG > NC_000913/1453445‑1453708 | cgccgcACGTTCGATAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCACTAATACCGTGGCGAATCAAAc > 1:268010/1‑140 (MQ=255) gccgcACGTTCGATAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACg > 1:654736/1‑140 (MQ=255) gccgcACGTTCGATAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACg > 1:446731/1‑140 (MQ=255) gcgtttCGATAAAGGTCATAGCGCGAAGGGGGGGGGCGCCTTTTACAATGGCAAACGGGGGGGGAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTa < 2:133633/136‑1 (MQ=255) cACGTTCGTTAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTa > 2:526979/1‑140 (MQ=255) cACGTTCGATAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTa > 2:574576/1‑140 (MQ=255) cACGTTCGATAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCAAAACACCTCGCCGCTAATAGCGCGGCGAATCAAACGGCTa > 2:236129/1‑140 (MQ=255) tCGATAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGcc > 2:442212/1‑140 (MQ=255) aTAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCg > 1:27409/1‑140 (MQ=255) aaGGTCATAGCGCGAGGGGGGGGGGCCCTTTTTTCAATGGCAACCGGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGcc < 2:31201/140‑1 (MQ=255) gTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGa > 1:662239/1‑140 (MQ=255) ggcgcGAGGGGGGGGGGCTCTTTTTTAAATGGAAAACTGGGGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGcc < 2:662239/139‑1 (MQ=255) gAAGGGCGGGGGCACATTTTTAGATCGCACACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTa < 1:312706/140‑1 (MQ=255) aaGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAGTAGCGTGGGTAAGCAAACGGCTACGGCCCCTGCCAGACTGCCAGGTAc > 2:179875/1‑140 (MQ=255) ccTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTAAGGCCCCGGCCAAACGGCCAGGTACCGGATAAGAAACt > 1:328201/1‑140 (MQ=255) tttCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGACCCCGGCGCGACTGCCTGGTACCGGATAAGAAACTGGCt > 2:247454/1‑140 (MQ=255) ggCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAActgct > 2:126621/1‑140 (MQ=255) aCTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATc > 2:66263/1‑140 (MQ=255) tGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGc < 1:372923/140‑1 (MQ=255) acccgccATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTcc < 1:574576/138‑1 (MQ=255) aGCCGCCATATCAAGACCTTCAGTGGTCACTTCCCATCACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTcc < 1:236129/140‑1 (MQ=255) ccATATCAAGACCTTCACTGGTAACCTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATg < 1:206343/140‑1 (MQ=255) aGACCTTCACTGGTCCCTTCCCCTACCGCGTCGCCGCGAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACAt < 1:205841/140‑1 (MQ=255) aCCTTCACTGGTCACCTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACAttt < 1:247454/140‑1 (MQ=255) ttCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGACACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTg < 2:429543/140‑1 (MQ=255) tCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCGTCGCTACTCTCCAAATGTTTCACATTTCTGt > 2:236420/1‑140 (MQ=255) tCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGt > 1:704/1‑140 (MQ=255) tCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGt > 2:115001/1‑140 (MQ=255) cACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGtt > 1:389285/1‑140 (MQ=255) tGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCt < 2:446731/140‑1 (MQ=255) gTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGGTAACTGCTGCATCGCTACTCTCCAGGTGTTGCACATTTCTGTTGCTaa > 2:267683/1‑140 (MQ=255) gTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTaa > 1:597301/1‑140 (MQ=255) gTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTaa > 1:422609/1‑140 (MQ=255) gTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTaa > 1:475082/1‑140 (MQ=255) ttCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCt > 2:530980/1‑133 (MQ=255) ttCCCATAACGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCt < 1:530980/133‑1 (MQ=255) aCGCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATcgcg < 1:442212/140‑1 (MQ=255) gCCTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGaa > 1:34475/1‑140 (MQ=255) cTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGaa > 2:441976/1‑138 (MQ=255) cTCGCCGCTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGaa < 1:441976/138‑1 (MQ=255) ccgcTAATAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATaa < 2:422609/140‑1 (MQ=255) aTAGCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCa < 1:115001/140‑1 (MQ=255) gCGTGGCGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAg > 2:536487/1‑140 (MQ=255) | CGCCGCACGTTCGATAAAGGTCATAGCGCGAAGGGCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCAGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAG > NC_000913/1453445‑1453708 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |