| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,460,012 | T→C | V320A (GTT→GCT) | paaH → | 3‑hydroxyadipyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,460,012 | 0 | T | C | 97.1% | 118.5 / NA | 35 | V320A (GTT→GCT) | paaH | 3‑hydroxyadipyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (17/17); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCAAGAAAAGTGGGCTGGGCGTGTACGACTGGCGCGCGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCC > NC_000913/1459876‑1460135 | ggCAAGAAAAGTGGGCTGGGCGTGTACGACTGGCGCGCGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGTAAATAAAGGTGACGGTTTCACGGAAATTGACGATGcttt > 2:202423/1‑140 (MQ=255) aaaGTGGGCTGGGCGTGTACGACTGGCGCGCGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTattgatt < 2:476383/140‑1 (MQ=255) gTGGGCTGGGCGTGTACGACTGGCGCGCGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGCAATTGACGAGGCTTTATTGATTgag > 1:286838/1‑140 (MQ=255) tgggctgggcGTGTACGACTGGCGCGCGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTgaga > 1:164454/1‑140 (MQ=255) ggctgggcGTGTACGACTGGCGCGCGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGaca > 1:434781/1‑140 (MQ=255) ggctgggcGTGTACGACTGGCGCGCGGAACGTGAGCCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGaca < 2:284340/140‑1 (MQ=255) ggcGTGTACGACTGGCGCGCGGCACGTGAGGCAGTGGTTGGCCTGGAATCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAgg < 2:290961/140‑1 (MQ=255) aCTGGCGCGCGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGca < 1:343599/140‑1 (MQ=255) aCTGGCGCGCGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGca > 1:598050/1‑140 (MQ=255) aCTGGCGCGCGGAACATGAGGCAGTTGTTCGCCTGGATGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGca < 1:232938/140‑1 (MQ=255) gcgcgcGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTTTTGATTGAAACACGAGGCGAGACGGCACAgg > 2:261748/1‑140 (MQ=255) gcgcgcGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAgg > 1:618830/1‑140 (MQ=255) gcgcgcGGAACGAGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCAGGGAAATTGACGATGGTTTTGTGATTGATACACATGGCGAGACGGCACAgg > 2:318215/1‑140 (MQ=255) cgcgcgGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGgc < 2:370451/140‑1 (MQ=255) gttgttGGCCTGGAAGCGGTAATCGACAGTTCTAGGCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGc < 2:286838/140‑1 (MQ=255) ttgttgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGGACCGGCGCTTGCAATACGACTGGCa > 1:217899/1‑140 (MQ=255) ttgttgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTTGCAATACGACTGGCa > 1:247166/1‑140 (MQ=255) ttgttgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCa > 2:611165/1‑140 (MQ=255) ttgttgGCCTGGAAGCGGTAAGCGACAGTTTTACCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAACGCGAGACGGCACAGGCGCTGGCAATACGCCTGGCa > 2:142142/1‑140 (MQ=255) gttgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACg < 1:503153/140‑1 (MQ=255) gttgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACg < 2:598050/140‑1 (MQ=255) gttgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACg < 1:368674/140‑1 (MQ=255) gttgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACg < 1:25561/140‑1 (MQ=255) tgGCCTGGAAGCGGTCAGCGACAGTCTTAACCCAATGCAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGcc < 1:202423/140‑1 (MQ=255) tgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGcc < 1:499047/140‑1 (MQ=255) ggAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCggtgg > 1:456482/1‑140 (MQ=255) cGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTg < 2:434781/140‑1 (MQ=255) aGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGTATTACGACTGGCAAGCTCGGTGGGAGTGATCGATAAAAt > 1:226320/1‑140 (MQ=255) cAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATAGGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAg > 1:411926/1‑140 (MQ=255) cAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAg < 1:586862/140‑1 (MQ=255) aTGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAggg > 1:654595/1‑139 (MQ=255) tGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAggtg < 1:632162/140‑1 (MQ=255) tGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAggtg < 1:501254/140‑1 (MQ=255) aaaagaaaagTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCAtt > 1:102805/1‑140 (MQ=255) aagTGACGGTGTCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGAGGTGACCATtgctgctg > 2:277209/1‑140 (MQ=255) gtgtCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTg > 1:476548/1‑140 (MQ=255) gtgtCACGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTg > 1:112456/1‑140 (MQ=255) aCGGAAATTGACGATGCTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGGGAAccc > 2:522151/1‑140 (MQ=255) | GGCAAGAAAAGTGGGCTGGGCGTGTACGACTGGCGCGCGGAACGTGAGGCAGTTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCC > NC_000913/1459876‑1460135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |