Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,504,220	A→G	K168K (AAA→AAG)	insQ →	putative insertion element transposase InsQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,504,220	0	A	G	100.0%	83.2 / NA	24	K168K (AAA→AAG)	insQ	putative insertion element transposase InsQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/10);  total (14/10)

ACGGGTGTTGTGAAAAATGTCACTGTCAGCCAGTCCTGCGGTAAGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAAAAGCAGAA  >  NC_000913/1504086‑1504351                                                                                                                                       |                                                                                                                                    aCGGGTGTTGTGAAAAATGTCACTGTCAGCCAGTCCTGCGGTAAGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGc                                                                                                                                >  1:85603/1‑140 (MQ=255)   gggTGTTGTGAAAAATGACACTGTCAGCCAGTCATTCGGGAAGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCa                                                                                                                              <  2:287299/140‑1 (MQ=255)                    tCACTGTCAGCCAGTCCGGCGGAAAGTGGTATATCCGTAGTCAGACAGCAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGcaca                                                                                                             <  2:217546/140‑1 (MQ=255)                           cAGCCAGTCCTGCGGTAAGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTg                                                                                                      <  1:137148/140‑1 (MQ=255)                                          tAAGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGtt                                                                                       <  2:566502/140‑1 (MQ=255)                                            aGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGtttt                                                                                     >  1:364624/1‑140 (MQ=255)                                                   cATCAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGaaaa                                                                              >  2:535726/1‑140 (MQ=255)                                                      cAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGTACAGTCTATGAGCCAGTAAACTGTTTTCAGAAAAAcc                                                                           >  1:216781/1‑140 (MQ=255)                                                      cAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCAGAGTATTTGAGCCGGTAAACAGTTTTCTGAAAAAcc                                                                           >  2:313314/1‑140 (MQ=255)                                                        gTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCag                                                                         >  2:564894/1‑140 (MQ=255)                                                              agacagaAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGaagaag                                                                   >  1:346955/1‑140 (MQ=255)                                                                   gaAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGc                                                              >  1:110618/1‑140 (MQ=255)                                                                        tGAAGTATCAACTCCGGCTCACACTTCAGCACCAAGGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGAc                                                         <  2:216781/140‑1 (MQ=255)                                                                          aaGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACtt                                                       >  2:343463/1‑140 (MQ=255)                                                                          aaGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACtt                                                       >  2:449370/1‑140 (MQ=255)                                                                              aTCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGc                                                   <  2:663969/140‑1 (MQ=255)                                                                                  aCTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGACTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAACCCAGAAGAAGCTGGCGAGACTTCAGCGACa                                               >  1:582130/1‑140 (MQ=255)                                                                                           cACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGc                                                   <  1:121528/127‑1 (MQ=255)                                                                                           cACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGc                                                   >  2:121528/1‑127 (MQ=255)                                                                                           cACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGCGACAGTTAAGCCg                                      <  1:449370/140‑1 (MQ=255)                                                                                                  cAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTc                               <  2:110618/140‑1 (MQ=255)                                                                                                                   cTGGATGCTGGCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGCGACAGTTAAGCTGCTAGGTCAAATTCAGCAACAACTg              >  1:272746/1‑140 (MQ=255)                                                                                                                            ggCGTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAAAAGCa     >  2:633199/1‑140 (MQ=255)                                                                                                                               gTGGCTAAGCTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAAAAGCAGaa  <  2:66654/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    ACGGGTGTTGTGAAAAATGTCACTGTCAGCCAGTCCTGCGGTAAGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAAAAGCAGAA  >  NC_000913/1504086‑1504351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑