Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,518,956	G→A	intergenic (‑110/‑71)	mnaT ← / → curA	L‑amino acid N‑acyltransferase/NADPH‑dependent curcumin/dihydrocurcumin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,518,956	0	G	A	100.0%	153.6 / NA	45	intergenic (‑110/‑71)	mnaT/curA	L‑amino acid N‑acyltransferase/NADPH‑dependent curcumin/dihydrocurcumin reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/17);  total (28/17)

CGGCTTTGCGGGCAAAACGGATGGACATGTCTGGCCTCAATAAAATAATGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGGCTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAAGCAGCGTAATCGACGTTGGGTTCTGGCCTCGCGTCCACATGGCG  >  NC_000913/1518819‑1519084                                                                                                                                          |                                                                                                                                 cGGCTTTGCGGGCAAAACGGATGGACATGTCTGGCCTCAATAAAATAATGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTTGATCAAATTATTGCGTGTTGCATAGGGGATGTGACGACt                                                                                                                                >  2:306001/1‑140 (MQ=255) cGGCTTTGCGGGCAAAACGGATGGACATGTCTGGCCTCAATAAAATAATGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACt                                                                                                                                >  2:153400/1‑140 (MQ=255)                     aTGGACATGTCTGGCCTCAATAAAAGAATCATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGt                                                                                                            <  1:262637/140‑1 (MQ=255)                          cATGTCTGGCCTCAATAAAATAATGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGcc                                                                                                       >  1:164304/1‑140 (MQ=255)                          cATGTCTGGCCTCAATAAAATAATGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGcc                                                                                                       >  1:84332/1‑140 (MQ=255)                             gTCTGGCCTCAATAAAATAATGGTGTTGAAGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGc                                                                                                    <  1:306001/140‑1 (MQ=255)                                 ggCCTCAATAAAATAATGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGctact                                                                                                <  1:575947/140‑1 (MQ=255)                                  gCCTCAATAAAATAATGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGctacta                                                                                               <  1:153400/140‑1 (MQ=255)                                       aataaaataaTGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGAt                                                                                          >  2:667768/1‑140 (MQ=255)                                       aataaaataaTGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGAt                                                                                          >  1:488802/1‑140 (MQ=255)                                                 tgatgatgatgTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGc                                                                                >  1:370170/1‑140 (MQ=255)                                                 tgatgatgatgTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGc                                                                                >  2:509749/1‑140 (MQ=255)                                                   atgatgatgTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCGATCCCGTGAGCGTGATACTATGGATGGATTCATGCCa                                                                              >  1:179994/1‑140 (MQ=255)                                                   atgatgatgTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCa                                                                              >  1:116073/1‑140 (MQ=255)                                                    tgatgatgTTATCAATATTATCCAAAACAGCCCGACGCACCGGATTCCGCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCaa                                                                             <  2:198665/140‑1 (MQ=255)                                                    tgatgatgTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCaa                                                                             <  2:381443/140‑1 (MQ=255)                                                    tgatgatgTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCaa                                                                             <  2:116073/140‑1 (MQ=255)                                                    tgatgatgTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCaa                                                                             <  2:23883/140‑1 (MQ=255)                                                       tgatgTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAg                                                                          >  1:424295/1‑140 (MQ=255)                                                              aTCAATACTATCGAAAACAGCCTGAAGCAACTGATACCTTGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGga                                                                   <  2:205986/140‑1 (MQ=255)                                                              aTCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGga                                                                   <  2:512092/140‑1 (MQ=255)                                                                    aCTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGAAGGAGTCTTGCCAAGAGCACAGGAGAGCAt                                                             >  1:169188/1‑140 (MQ=255)                                                                      tATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACAATTGATGCATTAATACCAAAATCACAGGAGAGCATTa                                                           >  1:202408/1‑140 (MQ=255)                                                                        tCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATg                                                         >  1:81465/1‑140 (MQ=255)                                                                        tCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATg                                                         >  2:367728/1‑140 (MQ=255)                                                                        tCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATg                                                         >  1:519043/1‑140 (MQ=255)                                                                        tCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATg                                                         >  2:130976/1‑140 (MQ=255)                                                                        tCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTAATGATGGAGTCAAGCAAAAAGCGCAGGAGAGCATTATg                                                         >  1:205986/1‑140 (MQ=255)                                                                          gAAAACAGCCCGAAGCAGCGGAATCCTCGATGAAATTATAGCGTGTTGAAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATggg                                                       <  2:259834/140‑1 (MQ=255)                                                                          gAAAACAGCCCGAAGCACCGGATTCCTCGATAAAAGTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATggg                                                       <  2:202408/140‑1 (MQ=255)                                                                          gAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATggg                                                       <  2:424295/140‑1 (MQ=255)                                                                           aaaaCAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATgggg                                                      >  1:543159/1‑140 (MQ=255)                                                                           aaaaCAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCA‑TATGGGGc                                                     >  2:31152/1‑140 (MQ=255)                                                                                  cccGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCGCAGGAGAGCGTTATGGGGCAACaaa                                               >  1:259834/1‑140 (MQ=255)                                                                                  cccGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACaaa                                               >  2:65793/1‑140 (MQ=255)                                                                                  cccGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACaaa                                               >  1:503886/1‑140 (MQ=255)                                                                                       aGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAagcag                                          <  2:438246/140‑1 (MQ=255)                                                                                              gATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAAGCAGCGTAATc                                   <  2:543159/140‑1 (MQ=255)                                                                                                      gATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAAGCAGCGTAATCGACGTTgg                           >  2:68443/1‑140 (MQ=255)                                                                                                        tGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAAGCAGCGTAATCGACGTTGGGt                         >  1:445011/1‑140 (MQ=255)                                                                                                        tGAAATTATTGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGAACAGGAGACCATCATGGGGCAACAAGAGCAACGTAATCGACGTTGGGt                         >  2:284243/1‑140 (MQ=255)                                                                                                              tattGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAAGCAGCGTAATCGACGTTGGGTTCTGGc                   <  1:633542/140‑1 (MQ=255)                                                                                                                ttGCGTGTTGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAAGCAGCGTAATCGACGTTGGGTTCTGGCCt                 <  1:611853/140‑1 (MQ=255)                                                                                                                        tGCAGAGGGGATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAAGCAGCGTAATCGACGTTGGGTTCTGGCCTCGCGTCca         >  1:389435/1‑140 (MQ=255)                                                                                                                               gggATGTGACGACTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAAGCAGCGTAATCGACGTTGGGTTCTGGCCTCGCGTCCACATGgcg  >  2:363086/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                 CGGCTTTGCGGGCAAAACGGATGGACATGTCTGGCCTCAATAAAATAATGATGATGATGTTATCAATACTATCGAAAACAGCCCGAAGCAACGGATTCCTCGATGAAATTATTGCGTGTTGCAGAGGGGATGTGACGGCTGCAAACAAAACCAATCCCGTGAGCCTGCTACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGGCAACAAAAGCAGCGTAATCGACGTTGGGTTCTGGCCTCGCGTCCACATGGCG  >  NC_000913/1518819‑1519084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑