Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,521,536	A→G	G510G (GGT→GGC)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,521,536	0	A	G	100.0%	69.0 / NA	21	G510G (GGT→GGC)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/11);  total (10/11)

TGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCACCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCA  >  NC_000913/1521407‑1521672                                                                                                                                  |                                                                                                                                         tGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGGGAGCCTAAGCAGCGCATCGCCAATAcgcg                                                                                                                                >  1:183344/1‑140 (MQ=255)         cAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTg                                                                                                                        <  2:85045/140‑1 (MQ=255)         cAGCGCCTTGACGACAGGTCTTACCAGCACTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTg                                                                                                                        <  1:285829/140‑1 (MQ=255)           gcgcCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGcc                                                                                                                      <  2:128224/140‑1 (MQ=255)                 tGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGAtctc                                                                                                                >  2:343097/1‑140 (MQ=255)                      acgGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGAtctc                                                                                                                <  1:525427/135‑1 (MQ=255)                      acgGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGAtctc                                                                                                                >  2:525427/1‑135 (MQ=255)                                                          aCCGCTAATGCTATCGACAGCAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATAc                                                                       >  2:387210/1‑140 (MQ=255)                                                                      aTCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCAt                                                           >  2:452797/1‑140 (MQ=255)                                                                         gACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAGACCGAAGTTCATAGCGCTTTGCCCATCAg                                                        >  1:307561/1‑140 (MQ=255)                                                                         gACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAg                                                        <  1:452797/140‑1 (MQ=255)                                                                         gACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAg                                                        <  2:156976/140‑1 (MQ=255)                                                                         gACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGACGGTTTTAAACCTAAGTTCATAGCGCTTTGCCCATCAg                                                        >  1:250988/1‑140 (MQ=255)                                                                                   tcatcatcaGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGa                                              <  1:20756/140‑1 (MQ=255)                                                                                   tcatcatcaGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGa                                              <  1:640748/140‑1 (MQ=255)                                                                                   tcatcatcaGTGTCGGGGTGAAACAATGCGATACTAAGCTGCCCATCGGCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGa                                              <  2:307561/140‑1 (MQ=255)                                                                                                             tGCGAGACTAAGCAGCCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCTGCACGATTAGAAAGATCATTACTCGTCGGCGTTTCaa                    >  1:210296/1‑140 (MQ=255)                                                                                                                       agcagcCCATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGccc          >  2:76469/1‑140 (MQ=255)                                                                                                                            cccATCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTg     >  1:466393/1‑140 (MQ=255)                                                                                                                               aTCGCCAATACGCGTTTTACTGCCGATCTCAATTGTATCCTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCa  <  2:244741/140‑1 (MQ=255)                                                                                                                               aTCGCCAATACGCGTTTTACTGACGATCTCACTTGCATCCTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCa  <  2:183344/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         TGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCATCAGTGTCGGTCTGAAACAATGCGAGACTAAGCAGCCCATCACCAATACGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCA  >  NC_000913/1521407‑1521672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑