Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,555,703	G→A	intergenic (‑34/+123)	maeA ← / ← sra	malate dehydrogenase, NAD‑requiring/30S ribosomal subunit protein S22

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,555,703	0	G	A	100.0%	53.6 / NA	17	intergenic (‑34/+123)	maeA/sra	malate dehydrogenase, NAD‑requiring/30S ribosomal subunit protein S22
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/9);  total (8/9)

TGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACT  >  NC_000913/1555582‑1555813                                                                                                                          |                                                                                                               tGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTg                                                                                              >  1:106216/1‑140 (MQ=255)             acaacGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCAGCCCTGGAGGGCGTTTATCGTCATTGCTTTACCGCCAg                                                                                  >  1:323154/1‑140 (MQ=255)                 cGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCg                                                                              <  2:57144/140‑1 (MQ=255)                    aaaTTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCg                                                                           >  1:475993/1‑140 (MQ=255)                      aTTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc                                                                         <  1:589190/140‑1 (MQ=255)                      aTTCCAGCAGTACAGCGCCAGCGTAAGGGCTATAAAGGGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc                                                                         <  1:211468/140‑1 (MQ=255)                         ccAGCAGTACAGGGCCAGCGTAAGGCATACAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTTCTTTACCGCCAGGGCGTCGGCCTc                                                                      <  1:203946/140‑1 (MQ=255)                                  cAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTCACCGCCAGGGCGTCGGCCTCAAGTATaaa                                                             >  1:260736/1‑140 (MQ=255)                                         aGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATa                                                      <  1:379091/140‑1 (MQ=255)                                          gCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATaa                                                     >  1:77263/1‑140 (MQ=255)                                          gCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATaa                                                     >  1:90301/1‑140 (MQ=255)                                                        aaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCa                                       <  1:554287/140‑1 (MQ=255)                                                            gAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTgc                                   >  1:129188/1‑140 (MQ=255)                                                             aaCGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTgcg                                  <  1:370459/140‑1 (MQ=255)                                                              aCGCTGTTTTTTTGTGTTTGGTTCCGTGTAACTCACTTTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTgcgc                                 <  1:221839/140‑1 (MQ=255)                                                               cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCa                                <  1:617431/140‑1 (MQ=255)                                                                                             ctcactcTTTTTTGAATATCCATCCCTGGAGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACt  >  2:470323/1‑140 (MQ=255)                                                                                                                          |                                                                                                               TGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACT  >  NC_000913/1555582‑1555813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑