Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,558,766	T→C	K84K (AAA→AAG)	ddpD ←	putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,558,766	0	T	C	100.0%	48.7 / NA	15	K84K (AAA→AAG)	ddpD	putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/8);  total (7/8)

TTGGTTGATGATGGCGGATCACGTCCATCATCTGAAGACCTATTCGACGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGTTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCAATAACTGCCCGTCGGTAGCAGACGCATAATCAGCATTGCG  >  NC_000913/1558645‑1558865                                                                                                                          |                                                                                                    ttGGTTGATGATCGCGGATCACGTCCATCATCTGAAGACCTATTCGACGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGa                                                                                   <  1:59714/140‑1 (MQ=255)            tgGCGGATCACGTCCATCATCTGAAGACCTATTCGACGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTcc                                                                        <  2:108646/140‑1 (MQ=255)            gggCGGATCACGTCCATCATCTGAAGGCCTATTCGTCGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGAGCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTcc                                                                        <  2:92864/139‑1 (MQ=255)                               tcTGAAGACCTATTCGACGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGcccc                                                     <  1:545335/140‑1 (MQ=255)                                   aaGACCTATTCGACGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAAAACCTCTTCTCCCAGCAGGGAAATCTGCCCCCGAt                                                 >  2:255092/1‑140 (MQ=255)                                          aTTCGACGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCa                                          >  1:382973/1‑140 (MQ=255)                                          aTTCGACGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCa                                          >  1:427633/1‑140 (MQ=255)                                                cGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAGTCAGCCCCCGATGTACGCAATAACt                                    >  1:308195/1‑140 (MQ=255)                                                cGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATg                                                <  1:413571/128‑1 (MQ=255)                                                cGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATg                                                >  2:413571/1‑128 (MQ=255)                                                     cGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCAATAACTGCCCg                               >  2:220033/1‑140 (MQ=255)                                                       gATTGAGGGCGGTCATCGGTTCTTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCAATAACTGCCCGTc                             <  1:220033/140‑1 (MQ=255)                                                                 ggTCATCGGTTCCTGAAAGATCGTCGCCACTCGTGGGCCGCGCCACTGGCGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCAATAACTGCCCGTCGGTAGCAGAc                   <  2:277130/140‑1 (MQ=255)                                                                  gTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCAATAACTGCCCGTCGGTAGCAGACg                  >  1:624445/1‑140 (MQ=255)                                                                                  aGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCAATAACTGCCCGTCGGTAGCAGACGCATAATCAGCATTGCg  <  1:639908/140‑1 (MQ=255)                                                                                  aGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGCTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCAATAACTGCCCGTCGGTAGCAGACGCATAATCAGCATTGCg  <  2:383693/140‑1 (MQ=255)                                                                                                                          |                                                                                                    TTGGTTGATGATGGCGGATCACGTCCATCATCTGAAGACCTATTCGACGTGTCGGATTGAGGGCGGTCATCGGTTCCTGAAAGATCATCGCCACTCGTGCGCCGCGCCACTGACGAAGCTGTTTTTCCCGCGCGTTAAGAACATCTTCTCCCAGCAGGGAAATCTGCCCCCGATGTACGCAATAACTGCCCGTCGGTAGCAGACGCATAATCAGCATTGCG  >  NC_000913/1558645‑1558865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑