| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,570,600 | T→C | intergenic (‑111/+45) | gadC ← / ← gadB | L‑glutamate:4‑aminobutyrate antiporter/glutamate decarboxylase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,570,600 | 0 | T | C | 97.1% | 113.5 / NA | 36 | intergenic (‑111/+45) | gadC/gadB | L‑glutamate:4‑aminobutyrate antiporter/glutamate decarboxylase B |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (15/19); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTC > NC_000913/1570465‑1570731 | tACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCg < 2:492215/140‑1 (MQ=255) ctgtctgtACTGATGTAGCCATTTTTTCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGaaa < 1:245899/140‑1 (MQ=255) gtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGtt < 1:69683/140‑1 (MQ=255) tAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAACGCCAGTGATCAGGCTTCGGAAATTGTCCCGAAACGGGTTCGTTTCggg > 2:236210/1‑140 (MQ=255) gCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGac > 2:616039/1‑140 (MQ=255) cATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGacac > 1:7204/1‑140 (MQ=255) tatCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACaccgtta < 1:295890/140‑1 (MQ=255) cTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTaa > 1:642839/1‑140 (MQ=255) cTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTaa < 2:448762/140‑1 (MQ=255) tAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTaa > 1:154361/1‑139 (MQ=255) tAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTaa < 2:154361/139‑1 (MQ=255) tATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCa > 1:57503/1‑140 (MQ=255) ttCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAgg < 2:475164/140‑1 (MQ=255) tCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGt < 1:509014/140‑1 (MQ=255) tCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGt < 1:441248/140‑1 (MQ=255) gTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGt > 2:381720/1‑140 (MQ=255) tCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGtt < 2:388137/140‑1 (MQ=255) gCACCTGTTTTGTTCTGAAGCGTATTCAGCACAATATCTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctg < 1:226935/140‑1 (MQ=255) cACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgt > 1:8230/1‑140 (MQ=255) tGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGctgttctg < 1:381720/140‑1 (MQ=255) tGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATa < 1:535391/140‑1 (MQ=255) aaGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATAcc > 2:26543/1‑140 (MQ=255) gCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCt < 2:7204/140‑1 (MQ=255) aCAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCggg > 2:544105/1‑140 (MQ=255) aaTATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTg > 1:599539/1‑140 (MQ=255) ttCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCt < 1:538048/140‑1 (MQ=255) cTAATGCCAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGCGCAATACTCTGAAGGTTCGGGCGATCGCTGAGATAtt > 1:209228/1‑140 (MQ=255) ccAGTGAATAGACTTTGGAAATTGCTCCGAACCGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGACAATGCCCTGCAGTTTCAGGTGATCGCTGAGATATTTCAggg < 2:480671/140‑1 (MQ=25) ccAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATGCCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAggg < 1:538173/140‑1 (MQ=255) ccAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAggg < 1:616039/140‑1 (MQ=255) ccAGTGAACAGACTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAggg < 2:642839/140‑1 (MQ=255) aGTGAACAGACTTTGGAAATTGACCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAg < 2:271311/140‑1 (MQ=255) aCTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAg > 2:438501/1‑140 (MQ=255) aCTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCAGATGATCGCTGAAATATTTCAGGGAGGCTTTGTAg > 2:296698/1‑140 (MQ=255) cTTTGGAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGt < 1:666803/140‑1 (MQ=255) ggAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTc > 2:537150/1‑140 (MQ=255) ggAAATTGCCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGGGATCGCTGAGATATTTCAGGGCGGCTTTGTAGTCTTc > 1:299995/1‑140 (MQ=255) | TACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTC > NC_000913/1570465‑1570731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |