| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,661,680 | G→A | W375* (TGG→TGA) | ynfF → | putative selenate reductase YnfF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,661,680 | 0 | G | A | 96.2% | 79.3 / NA | 26 | W375* (TGG→TGA) | ynfF | putative selenate reductase YnfF |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (14/11); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCTGGGCGAAGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGGGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAG > NC_000913/1661543‑1661810 | atTCTGGGCGAAGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTgagg > 2:534157/1‑140 (MQ=255) gggCGAAGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCcac > 1:139977/1‑140 (MQ=255) gggCGAAGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCcac > 2:469136/1‑140 (MQ=255) ggCGAAGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGTGATCGGTTGAGCTACACCTGCTTATATTTGATAGGGTTGAGGGCcaca > 2:287700/1‑140 (MQ=255) ggCGAAGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCcaca > 2:569561/1‑140 (MQ=255) ggCGAAGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCcaca > 1:643889/1‑140 (MQ=255) aGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAACCCTGCTTATATGTGCCAGGGTGGAGGGCCACAACGAc > 1:271168/1‑140 (MQ=255) tGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCa < 2:139977/140‑1 (MQ=255) cGGCATAGCTAAAACGCCGGAATGGGCAGC‑AAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACgg < 1:408119/140‑1 (MQ=255) cATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCg < 1:8599/140‑1 (MQ=255) ccGGAATGGGCAGCATAAATCACCAGCATACCGGCAGTAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCcg < 1:287700/140‑1 (MQ=255) ccGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCcg < 2:643889/140‑1 (MQ=255) ggAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTTTCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGTCCACAACGACACTCAAACGGCCAGCAAACATCCcgcg > 1:292148/1‑140 (MQ=255) aaaTCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGGGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCtt > 2:57413/1‑140 (MQ=255) aaTCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCttt < 1:605364/140‑1 (MQ=255) aaTCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCttt < 1:112035/140‑1 (MQ=255) ccAGCATCCCGGCAGAAAAAATCATCCAGTTTGCAGGAGCGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGtt < 2:271168/140‑1 (MQ=255) cATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCa > 2:477663/1‑140 (MQ=255) tCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGtt > 2:339818/1‑134 (MQ=255) tCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGtt < 1:339818/134‑1 (MQ=255) aCGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACt > 1:545207/1‑140 (MQ=255) cAAAACCTGCTTATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAggg > 2:531266/1‑139 (MQ=255) ttATATTTGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATc > 2:371583/1‑140 (MQ=255) tataTTTGTCAGGGTTGGGGGCTACAACGACATTCCAACAGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCt < 2:243739/140‑1 (MQ=255) ttGTCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTggggg < 1:617170/140‑1 (MQ=255) ttGTCAGGGTTGAGGGCCACAAAGACATTCCTACGTCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTggggg < 2:292892/140‑1 (MQ=255) tCAGGGTTGAGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAg > 1:444373/1‑140 (MQ=255) | ATTCTGGGCGAAGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAATTATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGGGGGCCACAACGACATTCCAACGGCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGCAACGTCGGCATAAACGGCGGCAACTCAGGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAG > NC_000913/1661543‑1661810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |