Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 130,378 A→G Y295H (TAC→CAC)  yacH ← DUF3300 domain‑containing protein YacH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913130,3780AG98.0% 167.2 / NA 50Y295H (TAC→CAC) yacHDUF3300 domain‑containing protein YacH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (27/22);  minor base C (1/0);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACGGTTGGGTTGTA  >  NC_000913/130242‑130513
                                                                                                                                        |                                                                                                                                       
atatTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTgcgtgcgt                                                                                                                                      <  2:98292/140‑1 (MQ=255)
        gttgtCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCaa                                                                                                                              >  1:622983/1‑140 (MQ=255)
        gttgtCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCaa                                                                                                                              <  1:421070/140‑1 (MQ=255)
           gtCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGc                                                                                                                           <  2:590077/140‑1 (MQ=255)
           gtCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGCCGTCGTCCAAGTTGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGc                                                                                                                           >  2:276740/1‑140 (MQ=255)
            tCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGcc                                                                                                                          <  1:30117/140‑1 (MQ=255)
            tCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGACGCTGCTGAATAGTGCGTGCGTGGTAGCAACGcc                                                                                                                          <  1:586603/140‑1 (MQ=255)
             cGCCGTTGTGTTGCCAGCCATTACCTTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGccc                                                                                                                         <  1:237983/140‑1 (MQ=255)
                  ttGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCt                                                                                                                    <  2:488657/140‑1 (MQ=255)
                   tgtgTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATCCTGATGATTAGTGCGTCCGTGTTAGCAACGCCCATGCta                                                                                                                   >  2:244398/1‑140 (MQ=255)
                          ccagccaTTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAATGCGTGCGTGGTAGCAAAGCCCATGCTATAGCCGa                                                                                                            >  2:269986/1‑140 (MQ=255)
                                     cGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACa                                                                                                 <  2:159058/140‑1 (MQ=255)
                                        cACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAg                                                                                              >  2:658371/1‑140 (MQ=255)
                                        cACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAg                                                                                              <  2:606198/140‑1 (MQ=255)
                                         aCGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGc                                                                                             >  1:587697/1‑140 (MQ=255)
                                                       cGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGt                                                                               >  2:63558/1‑140 (MQ=255)
                                                          ggtgATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTtct                                                                            <  2:432186/140‑1 (MQ=255)
                                                                gatAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCt                                                                      >  2:577330/1‑140 (MQ=255)
                                                                     tcatcaTTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCtggtgg                                                                 >  2:82327/1‑140 (MQ=255)
                                                                      catcatTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCtggtggt                                                                <  2:498700/140‑1 (MQ=255)
                                                                           ttGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAg                                                           >  1:354809/1‑140 (MQ=255)
                                                                           ttGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAg                                                           >  2:509703/1‑140 (MQ=255)
                                                                           ttGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAg                                                           >  2:609558/1‑140 (MQ=255)
                                                                              tCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATa                                                        >  2:632526/1‑140 (MQ=255)
                                                                                   atgatgatgGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACcg                                                   >  1:31647/1‑140 (MQ=255)
                                                                                   atgatgatgGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACcg                                                   >  1:426872/1‑140 (MQ=255)
                                                                                     gatgatgGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACcggc                                                 >  2:110061/1‑140 (MQ=255)
                                                                                       tgatgGTCATGATCGTCTTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACcggcgg                                               <  2:274574/140‑1 (MQ=255)
                                                                                       tgatgGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACcggcgg                                               <  1:577330/140‑1 (MQ=255)
                                                                                        gatgGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGa                                              >  1:571555/1‑140 (MQ=255)
                                                                                        gatgGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGa                                              >  2:457981/1‑140 (MQ=255)
                                                                                        gatgGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAACTGTCAACAAACGGATGACCGGCTGGTGGTGACAGATAAACCGGCGGa                                              >  2:312750/1‑140 (MQ=255)
                                                                                         atgGTCATGATCGTCGTCGTCCCAGTCGATTATCCGGAAAAGTGCGTGGGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGAt                                             <  2:309397/140‑1 (MQ=255)
                                                                                          tgGTCATGCTCGTCGTTCTCCCTGTCGATGTCGATCAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATa                                            <  2:310737/140‑1 (MQ=255)
                                                                                            gTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACg                                          <  1:570819/140‑1 (MQ=255)
                                                                                            gTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACg                                          <  1:82327/140‑1 (MQ=255)
                                                                                             tCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGc                                         >  1:611256/1‑140 (MQ=255)
                                                                                             tCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGc                                         <  1:30360/140‑1 (MQ=255)
                                                                                                tGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGAGGTAGCTGATAAATCGGCGGATACGCAGt                                      >  1:309397/1‑140 (MQ=255)
                                                                                                     gtcgtcgtcCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTgg                                 >  2:439402/1‑140 (MQ=255)
                                                                                                      tcgtcgtcCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGc                                >  1:51759/1‑140 (MQ=255)
                                                                                                           gtcCCAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCTCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGTCAGCTAAACCGGCGGATTAGCAGTATTGGCCCAGt                           >  2:264784/1‑140 (MQ=255)
                                                                                                             cccAGTCGATGCTGCTGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTc                         >  2:494184/1‑140 (MQ=255)
                                                                                                                       gctgctGAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCa               <  2:622983/140‑1 (MQ=255)
                                                                                                                          gctgAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACgg            <  1:110061/140‑1 (MQ=255)
                                                                                                                          gctgAATAGTGCGTGAGTGGTAGCATCGCCCATGCTAGAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACgg            <  1:264784/140‑1 (MQ=255)
                                                                                                                           ctgAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACggt           <  2:567845/140‑1 (MQ=255)
                                                                                                                            tgAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACggtt          >  2:583110/1‑140 (MQ=255)
                                                                                                                            tgAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACggtt          >  1:507941/1‑140 (MQ=255)
                                                                                                                            tgAATAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACggtt          >  1:344828/1‑140 (MQ=255)
                                                                                                                               aTAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACggttggg       <  2:587697/140‑1 (MQ=255)
                                                                                                                                tAGTGCGTGCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACggttgggt      >  1:16992/1‑140 (MQ=255)
                                                                                                                                   tgcgtgcgTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCGGTATTGGCCCAGTTGCCGTAACCCACGGTTGGGTTGt   >  1:212928/1‑140 (MQ=255)
                                                                                                                                   tgcgtgcgTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACGGTTGGGTTGt   >  2:377791/1‑140 (MQ=255)
                                                                                                                                   tgcgtgcgTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGAATTGGCCCAGTTGCCGTGAACCACGGTTGGGTTGt   >  2:181308/1‑140 (MQ=255)
                                                                                                                                    gcgtgcgtGGTAGCAGCGCCCAGGCCATACCCGAATCGGCGTACAAAGCTGTCATCAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACGGTTGGGTTGTa  <  1:269986/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACGGTTGGGTTGTA  >  NC_000913/130242‑130513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑