Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,666,271	T→C	G319G (GGT→GGC)	clcB →	putative chloride:H(+) antiporter ClcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,666,271	0	T	C	100.0%	106.3 / NA	31	G319G (GGT→GGC)	clcB	putative chloride:H(+) antiporter ClcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/16);  total (15/16)

CACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACA  >  NC_000913/1666135‑1666397                                                                                                                                         |                                                                                                                               cacCTGCAGTGTGGGGCAACGGCTATAGCACTGTAGAATGCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCggg                                                                                                                             <  2:221329/140‑1 (MQ=255)  acCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCgggg                                                                                                                            >  1:332006/1‑140 (MQ=255)         gtgtGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTTTGCCGTGCAGGCGAGTAGCGGTTCCAGCGCACCCGGCGGGGTCTTTac                                                                                                                     >  2:268331/1‑140 (MQ=255)          tgtgGGGCAACGGCTATAGCACCATACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTaca                                                                                                                    <  1:23129/140‑1 (MQ=255)            tgGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACAcc                                                                                                                  >  1:416943/1‑140 (MQ=255)             gggcCAACGGCTATAGCACCGTACAACCCTTTTTAGCCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAATCTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCg                                                                                                                 <  2:99716/140‑1 (MQ=255)                gCAACGGCTATAGCACCGTACACTCATTTCTAACCGCACCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACg                                                                                                              <  1:204098/140‑1 (MQ=255)                 cAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCTGCGGGGTCTTTACACCGACGc                                                                                                             <  2:472342/140‑1 (MQ=255)                        tataGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTAt                                                                                                      <  1:413996/140‑1 (MQ=255)                             cACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTc                                                                                                 <  2:49739/140‑1 (MQ=255)                                        cttttttAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCCCCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGc                                                                                      <  2:642449/138‑1 (MQ=255)                                        ccTTTTTAACCGCCCCATCACTGTTAATGATAACTGCCGCGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGc                                                                                      <  2:295529/140‑1 (MQ=255)                                        ccTTTTTAACCGCCCCACCACTATTCATGTTCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGc                                                                                      <  1:176674/140‑1 (MQ=255)                                                aCCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATTGGTCTTGCCATTGGCATGTTGTa                                                                              <  2:634945/140‑1 (MQ=255)                                                aCCGCCCCACCACTGTTAATGATCATTGCAGGGATCTCCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTa                                                                              <  1:195241/140‑1 (MQ=255)                                                   gCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGGAGCGGTTCCGGCGCACCCGGCGGGGACTTTACACAGACCCTACTTATCGGTATTGCCATTGGCATGTTGTAttg                                                                           >  2:221574/1‑138 (MQ=255)                                                     cccACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGTGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTc                                                                         <  1:558076/140‑1 (MQ=255)                                                              gTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGTCATTGGCATGTTGTATGGTCGTAGCCTgg                                                                <  2:384932/140‑1 (MQ=255)                                                               ttAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTggg                                                               >  2:111965/1‑140 (MQ=255)                                                                         ttGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTc                                                     >  1:113204/1‑140 (MQ=255)                                                                         ttGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGACTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTc                                                     >  1:149686/1‑140 (MQ=255)                                                                                       cTTTGTAAACTGTGTACCGTGCTGGTGAGGAGCGGTTACGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCgaaga                                       <  1:268331/140‑1 (MQ=255)                                                                                         ttGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGaaa                                     >  1:569146/1‑140 (MQ=255)                                                                                                cTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACt                              >  2:592372/1‑140 (MQ=255)                                                                                                  gtgtgCCGTGCTGGCGAGTAGCGGTTCCGACGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACttt                            >  1:148303/1‑140 (MQ=255)                                                                                                   tgtgCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGGTTATGGTTCCCTGATGGCGAAGAAATTACACtttt                           >  2:514697/1‑140 (MQ=255)                                                                                                     tgCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTAc                         >  2:29713/1‑140 (MQ=255)                                                                                                             tGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTg                 >  1:228112/1‑140 (MQ=255)                                                                                                             tGGCGAGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTGCCCTGATGGCGGAGGAATTACGCTTTTACTCGGATTg                 >  1:172582/1‑140 (MQ=255)                                                                                                                  aGTAGCGGTTCCGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCgg            <  1:514697/140‑1 (MQ=255)                                                                                                                            ccGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGCAATTACATTTTTACGCGGATTGACCGGGATGGCGaca  >  1:224222/1‑140 (MQ=255)                                                                                                                            ccGGCGCACCCGGCGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGaca  >  1:376438/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                               CACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACA  >  NC_000913/1666135‑1666397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑