Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,369	T→C	G271G (GGT→GGC)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,369	0	T	C	100.0%	51.1 / NA	16	G271G (GGT→GGC)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/11);  total (5/11)

GATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCA  >  NC_000913/1680241‑1680497                                                                                                                                 |                                                                                                                                 gATGTTGGCAAAGCGACTCTGCCGGCGGTTCCCTCTGCTTTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGa                                                                                                                       <  2:267788/140‑1 (MQ=255) gATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGa                                                                                                                       <  1:463835/140‑1 (MQ=255)  aTGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGa                                                                                                                              <  1:33079/132‑1 (MQ=255)  aTGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGa                                                                                                                              >  2:33079/1‑132 (MQ=255)      tGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAatga                                                                                                                  <  1:594982/140‑1 (MQ=255)       ggCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGGAACGCTGCTTTCGCTGGGGGTGGTGGCCCGTCCTGAACTGGCTGAAATTAGTAACCCCGCACTGACCGGCCTGATGGTGGAAatgat                                                                                                                 >  1:267788/1‑140 (MQ=255)               gACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCAt                                                                                                         <  2:346952/140‑1 (MQ=255)                        ggCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTTAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGaaa                                                                                                >  2:344763/1‑140 (MQ=255)                           ggTTCTCTCCGCGCCGGCCGCTTACTTGCTGGTAACGCTGGTTTCGCCGGGAGGGGCGGCAAGTCCCGAACTGGCTGAAATTGGTAACCCGCCCAGCGCCGGCCTGATGGTGGGAATGACGGGACCAAGTGGCCCAatcc                                                                                             >  2:648457/1‑139 (MQ=255)                                     gCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGc                                                                                   <  2:593678/140‑1 (MQ=255)                                     gCTCTGGGCGTTTACCTGCTGGTAACGCTGCTTTCGCCGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGc                                                                                   <  1:218356/140‑1 (MQ=255)                                          gggCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGtt                                                                              >  2:294314/1‑140 (MQ=255)                                           ggCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGttt                                                                             >  1:384477/1‑140 (MQ=255)                                                                  gctTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGcgcga                                                      >  2:503814/1‑139 (MQ=255)                                                                            ggTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTg                                            <  1:294314/140‑1 (MQ=255)                                                                                     gCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACcatcat                                   <  2:103096/140‑1 (MQ=255)                                                                                        cGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGgc                                <  2:384477/140‑1 (MQ=255)                                                                                          tCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTTAGCTGGACCATTATGgcag                              <  2:386716/140‑1 (MQ=255)                                                                                                                      tCAATGGCCGGCCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCa  >  2:586371/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                 GATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCA  >  NC_000913/1680241‑1680497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑