Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,682,372	T→C	S72P (TCT→CCT)	rstA →	DNA‑binding transcriptional regulator RstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,682,372	0	T	C	100.0%	90.0 / NA	26	S72P (TCT→CCT)	rstA	DNA‑binding transcriptional regulator RstA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/16);  total (10/16)

TACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCG  >  NC_000913/1682242‑1682508                                                                                                                                   |                                                                                                                                         tACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACGATTTGTCGTGAATTACGCGCATAGTGGCCTGGAcctg                                                                                                                                 >  1:258030/1‑138 (MQ=255) tACCGTAGAGCCGCGCGCCGACGAGGCCGAAGAAACCATGTTGCGAGATAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGa                                                                                                                                 <  2:202225/140‑1 (MQ=255)    cGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTg                                                                                                                              <  1:533439/140‑1 (MQ=255)     gTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGt                                                                                                                             <  2:355485/140‑1 (MQ=255)              cgcgGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAAc                                                                                                                    >  2:605800/1‑140 (MQ=255)                cggcgACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACct                                                                                                                  <  1:107738/140‑1 (MQ=255)                  gcgACCAGGCCGAAGAACCCATCTTGCGAGATAATTCGGCTTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACctct                                                                                                                <  2:198866/140‑1 (MQ=255)                               agaaACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGata                                                                                                   >  1:346528/1‑140 (MQ=255)                                    ccATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc                                                                                              >  1:493750/1‑140 (MQ=255)                                               gaAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGc                                                                                   >  1:507742/1‑140 (MQ=255)                                                aaaaTCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCa                                                                                  <  2:346528/140‑1 (MQ=255)                                                               tgtTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGcc                                                                   <  2:493750/140‑1 (MQ=255)                                                                 ttACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTg                                                                 <  2:507742/140‑1 (MQ=255)                                                                    cTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGa                                                              <  1:110456/140‑1 (MQ=255)                                                                        aCATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACtat                                                          <  2:72242/140‑1 (MQ=255)                                                                                      ggCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAacgac                                            >  2:618346/1‑140 (MQ=255)                                                                                       gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAacgacg                                           >  2:508660/1‑140 (MQ=255)                                                                                       gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAacgacg                                           >  2:516072/1‑140 (MQ=255)                                                                                       gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAacgacg                                           <  2:589706/140‑1 (MQ=255)                                                                                                         tttGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGcc                                         <  1:549968/124‑1 (MQ=255)                                                                                                         tttGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGcc                                         >  2:549968/1‑124 (MQ=255)                                                                                                         tttGTCGTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTa                         <  1:525414/140‑1 (MQ=255)                                                                                                               gTGATTTACGCGCAAAGTGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGt                   >  1:534810/1‑140 (MQ=255)                                                                                                                 gATTTACGCGCAAAGCGGCCTGCACCGATCGTTCTTCCAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGttt                 <  1:183739/140‑1 (MQ=255)                                                                                                                                tGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCg  <  1:516072/140‑1 (MQ=255)                                                                                                                                tGGCCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCg  <  1:508660/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         TACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGTTTTGCTAGCGCGTTTACGTTTGCATTTGCG  >  NC_000913/1682242‑1682508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑