Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,914	G→A	A180T (GCA→ACA)	gstA →	glutathione S‑transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,914	0	G	A	100.0%	103.8 / NA	32	A180T (GCA→ACA)	gstA	glutathione S‑transferase GstA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/13);  total (19/13)

AGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGT  >  NC_000913/1714789‑1715048                                                                                                                              |                                                                                                                                       aGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGt                                                                                                                          >  1:435074/1‑140 (MQ=255) aGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGt                                                                                                                          >  1:553819/1‑140 (MQ=255)   gCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTAt                                                                                                                        >  1:84166/1‑140 (MQ=255)         aaGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGa                                                                                                                  <  2:623715/140‑1 (MQ=255)                aGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCg                                                                                                           >  1:357771/1‑140 (MQ=255)                 gCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCgg                                                                                                          >  2:467573/1‑140 (MQ=255)                              gggCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGAcgc                                                                                             <  2:553819/140‑1 (MQ=255)                               ggCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGAcgcg                                                                                            >  1:140961/1‑140 (MQ=255)                               ggCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGAcgcg                                                                                            <  2:523981/140‑1 (MQ=255)                               ggCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGAcgcg                                                                                            <  2:151228/140‑1 (MQ=255)                                          aCAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGa                                                                                 <  2:346079/140‑1 (MQ=255)                                          aCAATTGCTGATGCCTACCTGTTTACGGTTCTGCGCTGGCCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGa                                                                                 <  2:277638/140‑1 (MQ=255)                                                gCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGTGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCgg                                                                                  >  1:564614/1‑133 (MQ=255)                                                gCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGTGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCgg                                                                                  <  2:564614/133‑1 (MQ=255)                                                           tCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAACTACAAAAGGCGCTGTCAGCGGAAGGCTTAAAGTAATAGc                                                                >  2:294568/1‑140 (MQ=255)                                                              gTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCAt                                                             <  1:467573/140‑1 (MQ=255)                                                                 tACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCgg                                                          >  1:564926/1‑140 (MQ=255)                                                                   cGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGcc                                                        >  1:114833/1‑140 (MQ=255)                                                                    ggTTCTGCGCTGGGCCTACGCGGTGAAACGGAATCTGCAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGccc                                                       <  1:294568/140‑1 (MQ=255)                                                                    ggTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGccc                                                       <  2:435074/140‑1 (MQ=255)                                                                         tGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAgg                                                  >  1:552220/1‑140 (MQ=255)                                                                                ggCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGACGGCTTAAAGTACTAACCATCGACCCACAGGTCGTtgt                                           >  1:215684/1‑140 (MQ=255)                                                                                ggCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTtgt                                           >  1:553361/1‑140 (MQ=255)                                                                                ggCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTtgt                                           >  2:625798/1‑140 (MQ=255)                                                                                     aCGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGcc                                      >  2:605870/1‑140 (MQ=255)                                                                                         ggTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGaaa                                  <  2:553361/140‑1 (MQ=255)                                                                                                   aaTCTGGAAGGGTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATAttt                        <  2:84166/140‑1 (MQ=255)                                                                                                             ggTTAGAGCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCAAAGGACGTAGTGGGCCGAAATGAGATATTTCAGAGCTttg              >  2:245582/1‑140 (MQ=255)                                                                                                                   agCACATTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTgcgc        <  1:331668/140‑1 (MQ=255)                                                                                                                        aTTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAg   >  1:92821/1‑140 (MQ=255)                                                                                                                        aTTGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCAGAACTGAGATATTTCAGTTCTTTGTTGCGCTGAAg   >  2:308602/1‑140 (MQ=255)                                                                                                                         ttGCAACATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGt  >  1:47889/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                       AGGCACTGAAGGATGAGCACTGGATCTGCGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGT  >  NC_000913/1714789‑1715048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑