Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,760,153	T→C	H124R (CAC→CGC)	sufS ←	L‑cysteine desulfurase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,760,153	0	T	C	100.0%	93.2 / NA	27	H124R (CAC→CGC)	sufS	L‑cysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/11);  total (16/11)

CAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTA  >  NC_000913/1760023‑1760284                                                                                                                                   |                                                                                                                                    cagGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCa                                                                                                                            >  2:120367/1‑140 (MQ=255)  agGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCAt                                                                                                                           <  2:18385/140‑1 (MQ=255)     cGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATctg                                                                                                                        <  2:540391/140‑1 (MQ=255)             ctcATCAAACAGCGTAGCCAGCCTCTCCAGTTGCAACGTCCCATCGGGTTTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACtgatga                                                                                                                <  2:190644/140‑1 (MQ=255)                                        cAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGc                                                                                     >  1:620605/1‑140 (MQ=255)                                               aaCGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGcc                                                                              <  2:620605/140‑1 (MQ=255)                                                      cATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTa                                                                       >  1:378678/1‑140 (MQ=255)                                                           ggATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGc                                                                  >  2:633267/1‑140 (MQ=255)                                                            gATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCg                                                                 >  1:145509/1‑140 (MQ=255)                                                             aTTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGcc                                                                              <  2:437568/126‑1 (MQ=255)                                                             aTTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGcc                                                                              >  1:437568/1‑126 (MQ=255)                                                                           cacGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTGGCTGTTGCCCCAGCAATTGGCTACCAGATTGATCCCt                                                  >  1:259545/1‑140 (MQ=255)                                                                                aGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCcgt                                             >  2:573242/1‑140 (MQ=255)                                                                                aGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCcgt                                             >  1:640552/1‑140 (MQ=255)                                                                                aGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTACTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCcgt                                             >  2:27541/1‑140 (MQ=255)                                                                                 gCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGAGACCAGATTGATCCCTTCcgtc                                            >  1:270827/1‑140 (MQ=255)                                                                                  ctctGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCcgtcg                                           >  2:550829/1‑140 (MQ=255)                                                                                  ctctGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTCGCAACCAGAATGATCCCTTCcgtcg                                           >  1:289463/1‑140 (MQ=255)                                                                                            aCGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGAc                                 >  2:428968/1‑140 (MQ=255)                                                                                            aCGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGAc                                 >  2:496030/1‑140 (MQ=255)                                                                                                   cgcAAAGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACAcc                          <  1:496030/140‑1 (MQ=255)                                                                                                        aGCATCTGCCAGGGAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGctc                     <  1:120367/140‑1 (MQ=255)                                                                                                                    ggAACAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCTGCGGACGAACACCAGCTCTTCCGCCGAACg         >  1:7773/1‑140 (MQ=255)                                                                                                                      aaCAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACggg       <  2:640552/140‑1 (MQ=255)                                                                                                                        cAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCa     <  1:633267/140‑1 (MQ=255)                                                                                                                        cAATGTTAGCGCGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCa     <  1:428968/140‑1 (MQ=255)                                                                                                                           tGTTAGCGCGGTACTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATta  <  1:27541/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    CAGGCGAGTTTTCTCATCAAACAGCGTAGGCAGCGTCTCCAGTTGCAACGTACCATCGGGATTGAGCGGGATCACACGCAGCTCTGCGCCAACGCGTGCGCAAAGCATCTGCCAGGGAACAATGTTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTA  >  NC_000913/1760023‑1760284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑