Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,764,553	T→C	intergenic (‑167/+160)	sufA ← / ← rydB	iron‑sulfur cluster insertion protein SufA/small regulatory RNA RydB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,764,553	0	T	C	100.0%	171.5 / NA	48	intergenic (‑167/+160)	sufA/rydB	iron‑sulfur cluster insertion protein SufA/small regulatory RNA RydB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

TAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGTATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAA  >  NC_000913/1764418‑1764677                                                                                                                                        |                                                                                                                             taACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCAtct                                                                                                                          <  1:35903/140‑1 (MQ=255)      gCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTg                                                                                                                     <  1:134412/140‑1 (MQ=255)       cATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGt                                                                                                                    >  2:599384/1‑140 (MQ=255)       cATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGt                                                                                                                    >  1:384426/1‑140 (MQ=255)              gTGATAATGATTATCAGTTCAACCCAGCATACGCAGGCGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATaa                                                                                                             <  1:219752/140‑1 (MQ=255)              gTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATaa                                                                                                             <  1:422078/140‑1 (MQ=255)                 aTAATGGTTATCTCTTCAACCCAGCAATCGCAGGTTCTTTACCGTAAAATATGCTTGTTGCCTTCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATgg                                                                                                          <  2:411683/140‑1 (MQ=255)                 aTAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTACGAAGTAAGCATCTGATTGTTAGATAATgg                                                                                                          >  1:277896/1‑140 (MQ=255)                 aTAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATgg                                                                                                          >  2:76663/1‑140 (MQ=255)                 aTAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATgg                                                                                                          >  2:529090/1‑140 (MQ=255)                 aTAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATgg                                                                                                          >  1:557855/1‑140 (MQ=255)                   aaTGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGtt                                                                                                        <  1:599384/140‑1 (MQ=255)                    aTGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGtta                                                                                                       >  2:48046/1‑140 (MQ=255)                        ttATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTtattat                                                                                                   >  2:329879/1‑140 (MQ=255)                           tCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTa                                                                                                <  1:529090/140‑1 (MQ=255)                              gTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAgg                                                                                             <  1:76663/140‑1 (MQ=255)                                tCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAg                                                                                           >  1:150584/1‑140 (MQ=255)                                 cAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGc                                                                                          >  2:376440/1‑140 (MQ=255)                                 cAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGc                                                                                          >  2:131076/1‑140 (MQ=255)                                       aGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAg                                                                                    >  1:95129/1‑140 (MQ=255)                                         cAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGc                                                                                  >  1:120379/1‑140 (MQ=255)                                            aCGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATTTAAGGAGCATTAAGGCtgt                                                                               >  1:610262/1‑140 (MQ=255)                                                  gggCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTa                                                                                      >  1:542727/1‑127 (MQ=255)                                                  gggCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTa                                                                                      <  2:542727/127‑1 (MQ=255)                                                         aCCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATgaaaaagaaa                                                                  <  1:150565/140‑1 (MQ=255)                                                         aCCGTAAAACATGCTGTTTGCCTGCTTTTAATAAGCAGAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATgaaaaagaaa                                                                  <  1:260430/140‑1 (MQ=255)                                                                    tGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACCCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATGTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTg                                                       >  1:423025/1‑140 (MQ=255)                                                                     gCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGa                                                      >  1:570396/1‑140 (MQ=255)                                                                           tGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGa                                                <  1:616607/140‑1 (MQ=255)                                                                           tGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGa                                                <  1:48046/140‑1 (MQ=255)                                                                            gCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGAc                                               <  1:56511/140‑1 (MQ=255)                                                                             ccTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACt                                              <  2:384426/140‑1 (MQ=255)                                                                                      aataaGCATAGACCCTGATGTGTGGTTTAACAGGCACCCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAagag                                     <  1:221554/140‑1 (MQ=255)                                                                                      aataaGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGa                                                <  1:370645/129‑1 (MQ=255)                                                                                      aataaGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGa                                                >  2:370645/1‑129 (MQ=255)                                                                                        taaGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGAt                                   <  1:654813/140‑1 (MQ=255)                                                                                                     tGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAACGAAAAAGTTGTATTGAAAATGACTATTTAAAAGATAGGTAAAAAAGTg                      >  2:291434/1‑140 (MQ=255)                                                                                                     tGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGAATTGAAACTGAATAGTTAAGAGATAGGTAAAAAAGTg                      >  1:246297/1‑140 (MQ=255)                                                                                                      gATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGc                     >  1:36902/1‑140 (MQ=255)                                                                                                      gATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGc                     >  1:494160/1‑140 (MQ=255)                                                                                                      gATGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAAAAAAAGTTGTATGGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGc                     >  2:192633/1‑140 (MQ=255)                                                                                                       aTGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCa                    <  2:570396/140‑1 (MQ=255)                                                                                                       aTGTGTGGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTACTGAAAATGACTGTTGAAGAGAGAGGTAAAAAAGTGCa                    >  1:194527/1‑140 (MQ=255)                                                                                                            tgGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGtt               <  2:557855/140‑1 (MQ=255)                                                                                                            tgGGTTAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGtt               >  1:584659/1‑140 (MQ=255)                                                                                                            tgGGTTAACAGGCACGCGAAGAATTAGGCATGTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGtt               <  2:277896/140‑1 (MQ=255)                                                                                                                ttAACAGGCACGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGa           <  2:530269/140‑1 (MQ=255)                                                                                                                         aCGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGaaa  <  2:584659/140‑1 (MQ=255)                                                                                                                         aCGCTAAGAATTAGGCATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGaaa  <  1:329879/140‑1 (MQ=255)                                                                                                                         aCGCTAAGAATTAGGCATCTCATTGTTAGATAATGCTTACTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGaaa  <  2:219490/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                             TAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCATAGACCCTGATGTGTGGGTTAACAGGCACGCTAAGAATTAGGTATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAA  >  NC_000913/1764418‑1764677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑