Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,766,131	T→C	N852S (AAC→AGC)	ydiJ ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,766,131	0	T	C	100.0%	50.9 / NA	16	N852S (AAC→AGC)	ydiJ	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/8);  total (8/8)

GTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGTTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACG  >  NC_000913/1766015‑1766258                                                                                                                     |                                                                                                                                gTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTc                                                                                                          <  2:464323/140‑1 (MQ=255)                          ctcGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaa                                                                                 <  1:549879/140‑1 (MQ=255)                             gCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAAc                                                                              <  1:450216/140‑1 (MQ=255)                             gCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAAc                                                                              <  1:664657/140‑1 (MQ=255)                             gCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAAc                                                                              <  1:94188/140‑1 (MQ=255)                                  gcgcCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTa                                                                         <  1:550753/140‑1 (MQ=255)                                                   tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGcc                                                        >  2:144631/1‑140 (MQ=255)                                                   tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGcc                                                        >  2:543122/1‑140 (MQ=255)                                                    cgcgATAACAAAGGACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCTAAAAGTTGAAGAAAACCTTTAATATGCTGGGCTTTGCCa                                                       >  2:292313/1‑140 (MQ=255)                                                                       cgcCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGATGGGCTTTGCCATGCGGCGAAAATGGCAGTa                                    >  2:315624/1‑140 (MQ=255)                                                                         ccGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAac                                  >  1:570036/1‑140 (MQ=255)                                                                          cGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAaca                                 <  1:543122/140‑1 (MQ=255)                                                                                         caTTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGACATCTGGCGAAAATGGAAGTAACACAGGCTGGAAACCTa                  >  2:263225/1‑140 (MQ=255)                                                                                                 tACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCGGTAGCACAGGCTGGAAACCTAATTTTTCg          >  1:183271/1‑140 (MQ=255)                                                                                                       gCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGa    <  2:605543/140‑1 (MQ=255)                                                                                                         ttCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACg  >  1:122437/1‑140 (MQ=255)                                                                                                                     |                                                                                                                                GTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGTTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACG  >  NC_000913/1766015‑1766258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑