Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	166,118	A→G	K463K (AAA→AAG)	mrcB →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	166,118	0	A	G	100.0%	91.5 / NA	27	K463K (AAA→AAG)	mrcB	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

CAGGCAAAACTGGGCGATAAGGTAAAAGATCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAACAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTGCGGGCTACAACCGTGCGATGCAGGCGCGTCGTTCGAT  >  NC_000913/165996‑166252                                                                                                                           |                                                                                                                                       cAGGCAAAACTGGGCGATAAGGTAAAAGATCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGa                                                                                                                       >  2:428465/1‑140 (MQ=255)      aaaaCTGGGCGATAAGGTAAAAGATCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTg                                                                                                                  >  1:564507/1‑140 (MQ=255)       aaaCTGGGCGATAAGGTAAAAGATCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGa                                                                                                                 <  1:428465/140‑1 (MQ=255)                  tAAGGTAAAAGATCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATtg                                                                                                      >  1:141945/1‑140 (MQ=255)                  tAAGGTAAAAGATCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATtg                                                                                                      <  2:562245/140‑1 (MQ=255)                             aTCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGAc                                                                                           <  2:624635/140‑1 (MQ=255)                               ctctcCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAGGCAGCGTAAGTTGAGCGATCTTGAAGCTGCTATTGTGGTCGTCGACCg                                                                                         >  2:197502/1‑140 (MQ=255)                                tctcCGGCGTGAAGATCTTCACTACCTTTGACTCGGTAGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGCCGACCGc                                                                                        >  2:124590/1‑140 (MQ=255)                                      gCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAgt                                                                                  >  2:408095/1‑140 (MQ=255)                                       cGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAgtg                                                                                 >  1:600726/1‑140 (MQ=255)                                              aTCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGt                                                                          <  2:585155/140‑1 (MQ=255)                                                           ttGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTc                                                             >  1:481263/1‑140 (MQ=255)                                                             gACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCgg                                                           >  2:532226/1‑140 (MQ=255)                                                                  ggTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGtt                                                      >  2:129426/1‑140 (MQ=255)                                                                   gTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACGGCTTTAGTGGTCAAATTCGTGAGATGGTCGGAGGTTc                                                     >  1:267180/1‑140 (MQ=255)                                                                   gTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTc                                                     >  1:404082/1‑140 (MQ=255)                                                                    tGGCCCAGGACGCGGCAGAAAAAGCCGCCGTAGAAGGCAGTCCGGCACGGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCt                                                    <  2:219932/140‑1 (MQ=255)                                                                                 ggCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTg                                       <  1:124590/140‑1 (MQ=255)                                                                                 ggCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTg                                       <  1:85819/140‑1 (MQ=255)                                                                                 ggCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTg                                       <  2:404082/140‑1 (MQ=255)                                                                                 ggCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTg                                       <  2:600726/140‑1 (MQ=255)                                                                                 ggCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTg                                       <  2:662391/140‑1 (MQ=255)                                                                                 ggCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGGTCGTGCGATGGTCGGAGATTCTGAGCCGCAGTTTg                                       >  2:272675/1‑140 (MQ=255)                                                                                              gccgTGGAAGGCATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTGCGGGCTACAACCg                          <  1:408095/140‑1 (MQ=255)                                                                                                         cATTCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTGGCGGGCTACAACCGTGCGATGCAgg               >  1:212762/1‑140 (MQ=255)                                                                                                            tCCGGCACTGAAGAAGCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCTGGAGTTTGCGGGGTACATCCGTGCGATGCAGgcgc            >  2:305055/1‑140 (MQ=255)                                                                                                                  aCTGAAGAAGCAGCGCAAGTTGCGCCATCGTGAAACTCCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTGCGGGCTACAACCGTGCGATGCAGGCGCGTCGtt      <  1:197502/140‑1 (MQ=255)                                                                                                                      aagaagCAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTGCGGGCTACAACCGTGCGATGCAGGCGCGTCGTTCGAt  >  1:562044/1‑140 (MQ=255)                                                                                                                           |                                                                                                                                       CAGGCAAAACTGGGCGATAAGGTAAAAGATCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCGCCGTGGAAGGCATTCCGGCACTGAAGAAACAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTTAGTGGTGAAGTTCGTGCGATGGTCGGAGGTTCTGAGCCGCAGTTTGCGGGCTACAACCGTGCGATGCAGGCGCGTCGTTCGAT  >  NC_000913/165996‑166252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑