Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,890,374	A→C	G53G (GGT→GGG)	yeaY ←	Slp family lipoprotein YeaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,890,374	0	A	C	100.0%	49.4 / NA	15	G53G (GGT→GGG)	yeaY	Slp family lipoprotein YeaY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/9);  total (6/9)

CATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAACCGC  >  NC_000913/1890238‑1890471                                                                                                                                         |                                                                                                  cATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCa                                                                                                <  1:473978/140‑1 (MQ=255)         cGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTTTACCGCAAACACTTTGGCACCAAAGAGTGCGTCCTGCCCAACGTACAg                                                                                        >  2:257654/1‑140 (MQ=255)           aCCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCt                                                                                      >  1:435114/1‑140 (MQ=255)               cgAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTa                                                                                           >  1:56458/1‑131 (MQ=255)               cgAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTa                                                                                           <  2:56458/131‑1 (MQ=255)                 agaAGGTTCTCCCAGTGTCGGTCTGGCTCCGCTGTCCGGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTgcggcg                                                                                <  1:257654/140‑1 (MQ=255)                 agaAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTgcggcg                                                                                <  2:435114/140‑1 (MQ=255)                             cAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTGCGGCGCACTCATCAccc                                                                    <  2:118558/140‑1 (MQ=255)                                  tCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTGCGGCGCACTCATCACCCGAACt                                                               <  1:468002/140‑1 (MQ=255)                                             ccGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCttgttg                                                    <  1:599487/140‑1 (MQ=255)                                                cTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTgcgg                                                 >  1:562206/1‑140 (MQ=255)                                                          gtacggtaCCAATTTCGCGGCGAGTTTTCCTCTCCTGGTTTTGTACCGTAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGActg                                       <  2:322249/140‑1 (MQ=255)                                                             cggtaGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGActgctg                                    >  2:48991/1‑140 (MQ=255)                                                                      tttCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATg                           <  2:507108/140‑1 (MQ=255)                                                                                               gTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGCCCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAACCGc  >  1:112477/1‑140 (MQ=255)                                                                                                                                         |                                                                                                  CATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCACAGTGACACAACCGC  >  NC_000913/1890238‑1890471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑