Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,900,027	G→A	intergenic (+2/+2)	pdeD → / ← yoaE	putative c‑di‑GMP phosphodiesterase PdeD/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,900,027	0	G	A	100.0%	162.4 / NA	49	intergenic (+2/+2)	pdeD/yoaE	putative c‑di‑GMP phosphodiesterase PdeD/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/32);  total (17/32)

TGGCGTGCATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCAT  >  NC_000913/1899890‑1900162                                                                                                                                          |                                                                                                                                        tGGCGTGCATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACt                                                                                                                                       <  2:40511/140‑1 (MQ=255)   gCGTGCATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTa                                                                                                                                     <  2:34488/140‑1 (MQ=255)        cATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTTATTTTCCCAAATGGCTGGCGGGCAACCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCAt                                                                                                                                >  2:556184/1‑140 (MQ=255)                         tttGTACGTACAGCCGGTGCCGGTACGTGAGCTTCTCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGt                                                                                                               <  2:314453/140‑1 (MQ=255)                         tttGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATTCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGt                                                                                                               <  2:422928/140‑1 (MQ=255)                         tttGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGt                                                                                                               <  2:655534/140‑1 (MQ=255)                         tttGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGt                                                                                                               <  2:548993/140‑1 (MQ=255)                         tttGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGt                                                                                                               <  1:330694/140‑1 (MQ=255)                         tttGTACGCACAGCCGATACCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGt                                                                                                               <  2:8737/140‑1 (MQ=255)                           tGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTg                                                                                                             <  2:510448/140‑1 (MQ=255)                               cGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGGTAACACTTACTCATATTCATCGTGCGCCGGTTGctct                                                                                                         >  1:213044/1‑140 (MQ=255)                                gCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGTCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGt                                                                                                               <  2:9429/133‑1 (MQ=255)                                gCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGTCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGt                                                                                                               >  1:9429/1‑133 (MQ=255)                                   caGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTaaa                                                                                                     <  1:338125/140‑1 (MQ=255)                                    aGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTaa                                                                                                     >  1:375678/1‑139 (MQ=255)                                    aGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTaa                                                                                                     <  2:375678/139‑1 (MQ=255)                                      ccGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATTACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACaa                                                                                                  >  2:435663/1‑140 (MQ=255)                                        gATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATg                                                                                                <  1:583037/140‑1 (MQ=255)                                        gATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATg                                                                                                <  1:578485/140‑1 (MQ=255)                                              gCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAAGACTTACTCATCTACATGGTGCGCCGGTTGCACTTTAACAATGCGAAcc                                                                                          >  2:302886/1‑140 (MQ=255)                                                tACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAg                                                                                        >  1:479623/1‑140 (MQ=255)                                                  cGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGAt                                                                                      <  1:132540/140‑1 (MQ=255)                                                    tGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCa                                                                                    <  2:479623/140‑1 (MQ=255)                                                      aTTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAac                                                                                  <  2:366975/140‑1 (MQ=255)                                                        tttCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGGGCCGGTTGCTCGTTGACAATGCGAACCAGATCAacac                                                                                >  1:309031/1‑140 (MQ=255)                                                          tCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGa                                                                              <  1:653254/140‑1 (MQ=255)                                                                 tggctggcGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCAt                                                                       <  2:105647/140‑1 (MQ=255)                                                                    ctggcGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTgg                                                                    >  2:65715/1‑140 (MQ=255)                                                                    ctggcGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTgg                                                                    >  2:583191/1‑140 (MQ=255)                                                                     tggcGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGc                                                                   <  2:355922/140‑1 (MQ=255)                                                                             aGCCAACCGCCGCCCGCCCGGCATAATGGACTTATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAatga                                                           <  2:169876/140‑1 (MQ=255)                                                                             aGCCAACCGCCGCCCGCCCGGCATAATGGACATATTACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAatga                                                           <  1:435663/140‑1 (MQ=255)                                                                                 aaCCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGt                                                       <  2:658787/140‑1 (MQ=255)                                                                                  aCCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTg                                                      <  2:10828/140‑1 (MQ=255)                                                                                    cgccgcCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGat                                                    <  2:591596/140‑1 (MQ=255)                                                                                           cgcccgGCATAATGGCCATATCCCGGCCAATATGCCGCTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGt                                             <  1:302886/140‑1 (MQ=255)                                                                                           cgcccgGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGt                                             >  1:125426/1‑140 (MQ=255)                                                                                               cgGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGcc                                         <  1:574810/140‑1 (MQ=255)                                                                                                   aTAATGGACATATCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTAc                                     <  2:482349/140‑1 (MQ=255)                                                                                                              atCACGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAAGCATTGGCTGCAAAGATCGTGATATGAAGTGCCCCTACATCAATCACAt                          >  2:262233/1‑140 (MQ=255)                                                                                                                  cGCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGct                      <  1:556184/140‑2 (MQ=255)                                                                                                                   gCCCATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGAAGGTGATAGGCATTGGCCCTACTTCAATCACATCGccc                     >  1:203266/1‑140 (MQ=255)                                                                                                                    cccATTATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCca                    >  1:164640/1‑140 (MQ=255)                                                                                                                      cATTATGCCGTTACGTTTACACTTACTCATCTTCAGCGTTCGCCGGTTTCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCcaca                  <  2:213044/140‑1 (MQ=255)                                                                                                                        ttATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACg                >  1:132689/1‑140 (MQ=255)                                                                                                                        ttATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACg                <  2:132689/140‑1 (MQ=255)                                                                                                                         tATGCCGTTACGTTAACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACgg               >  2:396723/1‑140 (MQ=255)                                                                                                                                  acgttaACACTTACTCATCTTCATCGTGTGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGa      >  1:6352/1‑140 (MQ=255)                                                                                                                                      taACACTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCAt  <  1:583191/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGGCGTGCATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCAT  >  NC_000913/1899890‑1900162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑