Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,965,121	T→C	G357G (GGA→GGG)	flhB ←	flagellar biosynthesis protein FlhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,965,121	0	T	C	100.0%	84.6 / NA	26	G357G (GGA→GGG)	flhB	flagellar biosynthesis protein FlhB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/14);  total (12/14)

TGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCATGTCGATACAGCGCTCGCGCCAGCGGC  >  NC_000913/1964998‑1965247                                                                                                                            |                                                                                                                               tgtgTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGcc                                                                                                                >  2:558433/1‑140 (MQ=255) tgtgTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGcc                                                                                                                >  1:558193/1‑140 (MQ=255)  gtgtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCa                                                                                                               >  1:153366/1‑140 (MQ=255)           tCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAg                                                                                                      <  2:153366/140‑1 (MQ=255)                tttGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGcc                                                                                                 <  2:514453/140‑1 (MQ=255)                  tGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAg                                                                                               >  1:566457/1‑140 (MQ=255)                  tGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGGTGTACAGGTCGCTGCCCACCAGCCAAACGCCAGCGTTTCAGTTGCCAg                                                                                               >  2:214335/1‑140 (MQ=255)                  tGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGGTGTACAGGCCGCTGCCCACCAGCCAAACGCCAGCGTTTCAGTTGCCAg                                                                                               >  2:214344/1‑140 (MQ=255)                   gCGGGCAGGCGCAGCATCGCGGCCAGATTACTTATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGa                                                                                              >  1:120018/1‑140 (MQ=255)                                   tCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTAAAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTc                                                                              <  2:533111/140‑1 (MQ=255)                                                aCTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCgcggcg                                                                 <  2:558193/140‑1 (MQ=255)                                                   cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGTCAACACTTCCGCCACCGCGGCGTAc                                                              >  1:263050/1‑140 (MQ=255)                                                      gggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGt                                                           >  1:388694/1‑140 (MQ=255)                                                      gggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGt                                                           <  1:514914/140‑1 (MQ=255)                                                            gTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGAccc                                                     >  1:116282/1‑140 (MQ=255)                                                                   gTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGAttt                                              <  1:88407/140‑1 (MQ=255)                                                                                  ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCg                               <  2:29677/140‑1 (MQ=255)                                                                                  ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCg                               <  1:158528/140‑1 (MQ=255)                                                                                  ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGCCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCg                               >  1:388453/1‑140 (MQ=255)                                                                                    cTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCa                             <  1:117985/140‑1 (MQ=255)                                                                                       ccGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCATGt                          >  2:413657/1‑140 (MQ=255)                                                                                            aCCGGAAGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCCCTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCATGTCGATa                     <  2:485182/140‑1 (MQ=255)                                                                                                  aGATGAGTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCATGTCGATACAgcgc               <  1:413657/140‑1 (MQ=255)                                                                                                   gATGAGTAGGTTGTACACGGCGGTGCCCACCAGCCGGACCCCAGCGTTGCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCATGTCGATACAGCGCt              <  2:263050/140‑1 (MQ=255)                                                                                                        gTAGGTTGTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCATGTCGATACAGCGCTCgcgc         <  1:558433/140‑1 (MQ=255)                                                                                                               gTACAGGGCGCTGCCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCATGTCGATACAGCGCTCGCGCCAgcggc  <  2:116282/140‑1 (MQ=255)                                                                                                                            |                                                                                                                               TGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCATGTCGATACAGCGCTCGCGCCAGCGGC  >  NC_000913/1964998‑1965247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑