Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	166,897	T→C	V723A (GTC→GCC)	mrcB →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	166,897	0	T	C	100.0%	116.4 / NA	37	V723A (GTC→GCC)	mrcB	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/16);  total (21/16)

CAACGCGGTACGGGTCGTCAGCTTGGGGCGAAATACCCGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTG  >  NC_000913/166764‑167033                                                                                                                                      |                                                                                                                                         cAACGCGGTACGGGTCGTCAGCTTGGGGCGAAATACCCGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGgccggccg                                                                                                                                    <  1:556274/140‑1 (MQ=255)     gcgGTACGGGTCGTCACCTTGGGGCGAAATACCCGGACCCGCATCTGGGAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGAt                                                                                                                                <  2:308940/140‑1 (MQ=255)                 gtcAGCTTGGGGCGAAATACCCGAACCTGCATCTGGCAAGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGAGCATAACCTGGGCCGGCAGTGATAGCAACCAGCCg                                                                                                                    >  1:418793/1‑140 (MQ=255)                  gcAGCTTGGGGCGAAATACCCGATCCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCTGGCATTGACGGCAGCACGGTGACCATAACCTGGGACGGTCGTGATAACAACCAGCCGa                                                                                                                   <  2:583420/139‑1 (MQ=255)                    aGCTTGGGGCGAAATACCCGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGAcc                                                                                                                 <  1:495515/140‑1 (MQ=255)                        tGGGGCGAAATACCCGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAAc                                                                                                             >  2:557781/1‑140 (MQ=255)                                aaTACCCGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGAcc                                                                                                                 <  1:137197/128‑1 (MQ=255)                                aaTACCCGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGAcc                                                                                                                 >  2:137197/1‑128 (MQ=255)                                         aCCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCggg                                                                                            >  1:448294/1‑140 (MQ=255)                                          ccTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTTATAACAATAAGCCGACAAAACCTTATGGTGACAGCgggg                                                                                           >  2:325077/1‑140 (MQ=255)                                                   ggCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGa                                                                                  >  2:441874/1‑140 (MQ=255)                                                       gggAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTa                                                                              <  1:342832/140‑1 (MQ=255)                                                        ggAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTAt                                                                             >  2:598484/1‑140 (MQ=255)                                                                gggACTTCCAACAATCACGTAACATGCTGAGTTGGGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTa                                                                     <  1:311423/140‑1 (MQ=255)                                                                gggACTACCAACAATGACGTAGATACCTGCTTTGCGGCCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTa                                                                     <  1:209271/140‑1 (MQ=255)                                                                  gACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTAAGGTGCCAGCGGGGCGATGTCGATTTATCAGCGTTATc                                                                   >  1:326104/1‑140 (MQ=255)                                                                          acaaTAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAAc                                                           <  1:398104/140‑1 (MQ=255)                                                                            aaTAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCATCGGCGCAATGTCGATTAATCAGCGTTATCTGGCTAACCa                                                         >  2:301714/1‑140 (MQ=255)                                                                               aaCGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGAc                                                      >  1:57975/1‑140 (MQ=255)                                                                                 cGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGc                                                    >  2:408240/1‑140 (MQ=255)                                                                                 cGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGc                                                    >  2:376356/1‑140 (MQ=255)                                                                                     gATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACTATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAAATGTATGGTGCCAGCGGGGCAATGTTGATTTATCAGCGTTATCTGGCTAACCAGACGCCAAc                                                >  1:453676/1‑140 (MQ=255)                                                                                     gATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGACGATTTACCAGTGTTATCTGGCTAACCAGACGCCAAc                                                >  1:261670/1‑140 (MQ=255)                                                                                         ccTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAAcgccg                                            <  1:301714/140‑1 (MQ=255)                                                                                          cTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAAcgccgc                                           >  1:577867/1‑140 (MQ=255)                                                                                            ggTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTTTCAGCGTTATCAGGCTAACCAAACGCCAACGCCGCTg                                         >  1:249384/1‑140 (MQ=255)                                                                                                  cGGGCATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCtt                                   >  2:502393/1‑140 (MQ=255)                                                                                                   gggCATTGACGGCAGCACGGTGACCATCACCTGCGCCGGCCGTGATAACAACCCCCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGTTTTATCAGCGTTATCTGGCTAACCAGACGCCCACGCCGCTGAATCTTg                                  >  2:486613/1‑140 (MQ=255)                                                                                                      cATTGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTc                               <  1:502393/140‑1 (MQ=255)                                                                                                        ttGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCg                             <  1:632743/140‑1 (MQ=255)                                                                                                         tGACGGCAGCACGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGc                            <  1:346624/140‑1 (MQ=255)                                                                                                               cagcaCGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaag                      >  1:580416/1‑140 (MQ=255)                                                                                                                agcaCGGTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                     <  2:448294/140‑1 (MQ=255)                                                                                                                    cGGTGACCATCACCTTGGCCGGTCGTGATTACAACCACCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATAtt                 <  1:325077/140‑1 (MQ=255)                                                                                                                     ggTGACCATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTg                >  1:363996/1‑140 (MQ=255)                                                                                                                     ggTGACCATCAACTCGGCCGGCCGTGATAACACCCACCCGACCAAACTGTATGGTGCCAGCCCGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTg                <  2:418793/140‑1 (MQ=255)                                                                                                                      gTGACCATCACCTGGGCCGGCCCTGATAACACCCACCCGCCCAAACTGAATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGc               <  2:171266/140‑1 (MQ=255)                                                                                                                           cATCACCTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGata          <  1:128062/140‑1 (MQ=255)                                                                                                                                 cTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCg    <  1:441874/140‑1 (MQ=255)                                                                                                                                 cTGGGCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGCTCCACCTGAAGATGTTGCAGATATGGGCg    >  1:307568/1‑140 (MQ=255)                                                                                                                                   gggCCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCCACGCCGCTGAATTTTGTTCCGCCAGGAGATTTTGCAGATATGGGCGTg  >  2:213978/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         CAACGCGGTACGGGTCGTCAGCTTGGGGCGAAATACCCGAACCTGCATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTG  >  NC_000913/166764‑167033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑