Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,028,495	A→G	V291A (GTG→GCG)	yedI ←	conserved inner membrane protein YedI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,028,495	0	A	G	100.0%	87.2 / NA	27	V291A (GTG→GCG)	yedI	conserved inner membrane protein YedI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

TAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACC  >  NC_000913/2028361‑2028623                                                                                                                                       |                                                                                                                                 tAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGGGTATATTCTCTACGGCTTCCACTCCCCGCGCCACg                                                                                                                             >  2:312095/1‑140 (MQ=255)         aTACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATgccgcc                                                                                                                     >  2:541002/1‑140 (MQ=255)         aTACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATgccgcc                                                                                                                     >  2:106496/1‑140 (MQ=255)                            gAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATc                                                                                                  <  1:525185/140‑1 (MQ=255)                             aGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACAGCTTTCACTCCGAGCGCCATGATACCTCCGATGTTAAATCCAAGAATCa                                                                                                 >  1:233025/1‑140 (MQ=255)                                        ttCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGGCCGCGCATTTTCTCTACGGCTTTCACTCCCAACGCCACGATGCCGCCGATGATAAATCCAAGAAGCAGATTTAAAACa                                                                                      <  2:478131/140‑1 (MQ=255)                                        ttCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACa                                                                                      <  1:459427/140‑1 (MQ=255)                                             cTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCgg                                                                                 >  2:158980/1‑140 (MQ=255)                                                          ggCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCGAAGAATCAGATTTAAAACAGTCGGTAATATCATCGcc                                                                    >  1:258966/1‑140 (MQ=255)                                                          ggCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGcc                                                                    <  1:106496/140‑1 (MQ=255)                                                          ggCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGcc                                                                    >  1:432148/1‑140 (MQ=255)                                                           gCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCa                                                                   >  2:127480/1‑140 (MQ=255)                                                            cGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCac                                                                  >  2:259788/1‑140 (MQ=255)                                                                             ccAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGcc                                                 <  1:259788/140‑1 (MQ=255)                                                                              cAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGccc                                                >  1:489069/1‑140 (MQ=255)                                                                                  gCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCg                                            <  1:127480/140‑1 (MQ=255)                                                                                    gAGTATTAATGTGCCTGCCCGCCCATTTTCTCTACGGCTCTCACTCCCTGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGaa                                          <  2:173714/140‑1 (MQ=255)                                                                                    gAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGaa                                          <  1:158980/140‑1 (MQ=255)                                                                                            aTGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCaa                                  <  2:489069/140‑1 (MQ=255)                                                                                               tgCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATgg                               >  2:415310/1‑140 (MQ=255)                                                                                                 ccTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCAATTTACTTCCCGGCTAAATGTTCAATGGCg                             >  1:277035/1‑140 (MQ=255)                                                                                                     aCCGCGCATTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGAt                         >  2:575025/1‑140 (MQ=255)                                                                                                            aTTTTCTCTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTGCTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAgcgg                  <  2:589203/140‑1 (MQ=255)                                                                                                                tctcTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCa              >  1:385294/1‑140 (MQ=255)                                                                                                                tctcTACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCa              >  2:60642/1‑140 (MQ=255)                                                                                                                 ctctACGGCTTTCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCaa             <  1:575025/140‑1 (MQ=255)                                                                                                                   ctACGGCTTTCACTCCCAGCGCCACGATGCCCCCGGTGAAAAATCCAAAAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCAAAATGTTCAATGGCGTGATGCAGCGGCGCAAtt           <  2:258966/140‑1 (MQ=255)                                                                                                                            tCACTCCCAGCGCCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTAcc  <  1:541002/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 TAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACC  >  NC_000913/2028361‑2028623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑