BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,077,169	T→C	W20R (TGG→CGG)	cbeA →	CP4‑44 prophage; cytoskeleton bundling‑enhancing antitoxin CbeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,077,169	0	T	C	100.0%	114.1 / NA	34	W20R (TGG→CGG)	cbeA	CP4‑44 prophage; cytoskeleton bundling‑enhancing antitoxin CbeA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/11); total (23/11)

AACTGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGACCAGG  >  NC_000913/2077033‑2077304

aaCTGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAAAACTACCCGACGACAATCACGACCGCCCCTGGCggg                                                                                                                                      >  2:223784/1‑140 (MQ=255)

  cTGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACCCTTCCCGACGACAATCACGACCGCCCCTGGCgggga                                                                                                                                    <  2:348056/140‑2 (MQ=255)

     cgTGGGAATTCTGATGAGCAGAATTATGGCCACTACCTCACCCTTCATAGCCGATTTTCTGTAAAGATTTTAGCGTGCCAGACACACACCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTg                                                                                                                                 >  2:332879/2‑140 (MQ=255)

     aGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTg                                                                                                                                 >  2:411374/1‑140 (MQ=255)

     aGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTg                                                                                                                                 >  1:603749/1‑140 (MQ=255)

      gTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGc                                                                                                                                >  2:571921/1‑140 (MQ=255)

      gTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGc                                                                                                                                >  1:366903/1‑140 (MQ=255)

          gAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTg                                                                                                                            >  1:365859/1‑140 (MQ=255)

                 gatgaGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAGTCACGACGGCCTCTAGCGGGGACTGCCCTGCACCGTg                                                                                                                     >  1:198926/1‑140 (MQ=255)

                      gCAGAATTATCGCCACTCCCGGCCCATTCTTTGTCAATTGTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGcc                                                                                                                <  2:306665/140‑1 (MQ=255)

                          aaTTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGt                                                                                                            >  1:336807/1‑140 (MQ=255)

                               tCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCgg                                                                                                       >  2:392559/1‑140 (MQ=255)

                                    aCTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCAc                                                                                                  >  1:33603/1‑140 (MQ=255)

                                      tACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGAATACGCTGCACCGAGACGCCCTGTTTCGGGGCACGt                                                                                                >  2:296536/1‑140 (MQ=255)

                                             ccATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGc                                                                                         <  1:475486/140‑1 (MQ=255)

                                                ttCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGGCCTGTTTCGGGGCACGCCTGGTGCagg                                                                                      >  2:369847/1‑140 (MQ=255)

                                                ttCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGCGACTGCCCGGCACCGTGACGCCCTGCTTCGCGGCACGTCTGGTGCagg                                                                                      >  2:236869/1‑140 (MQ=255)

                                                            tttCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGt                                                                          >  2:466050/1‑140 (MQ=255)

                                                                     ggATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTAcc                                                                 <  1:392559/140‑1 (MQ=255)

                                                                             tCGTGTCAGACACACCCCCCGGGACAACACTTCCCGAAGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGAc                                                         <  1:296536/140‑1 (MQ=255)

                                                                                 gtCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACcgcg                                                     >  1:432048/1‑140 (MQ=255)

                                                                                 gtCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTACAGGAGGATAAGCGCTTGCATCACCTTGCCGACcgcg                                                     >  1:222950/1‑140 (MQ=255)

                                                                                            tCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCaga                                          >  2:391135/1‑140 (MQ=255)

                                                                                                   gACAACACTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGt                                   >  2:398723/1‑140 (MQ=255)

                                                                                                    acaacaCTTCCCGACTACAATCCCGCCCGTCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGtt                                  <  1:223784/140‑1 (MQ=255)

                                                                                                     caacaCTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTc                                 <  1:391135/140‑1 (MQ=255)

                                                                                                       acacTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAg                               <  2:603749/140‑1 (MQ=255)

                                                                                                        cacTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCTACCACGGCGGTATCAGAGGCCTGTTCAGc                              >  1:245053/1‑140 (MQ=255)

                                                                                                        cacTTCCCGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGc                              >  2:457798/1‑140 (MQ=255)

                                                                                                             cccGACGACAATCACGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGc                         >  2:152656/1‑140 (MQ=255)

                                                                                                                     cAATCGCGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGt                 <  2:65824/140‑1 (MQ=255)

                                                                                                                           cGACCGCCCCTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTaccacc           <  2:336807/140‑1 (MQ=255)

                                                                                                                             aCCGCGCCTGGCGGGGACTGCCCTGCACCGTGACGCCATGTTTCGGGGCACGTCTGGGGCAGGAGGGTAACCGGTTGCATTACCTTGCCGAGCGCGCCGGCATCAGAGGCCTGTTCACCGATGCAGATGCGCACCACCTg         >  2:273060/1‑140 (MQ=255)

                                                                                                                                 ccccTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGAcc     >  2:17156/1‑140 (MQ=255)

                                                                                                                                    cTGGCGGGGACTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGACCAgg  <  1:152656/140‑1 (MQ=255)

AACTGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGACCAGG  >  NC_000913/2077033‑2077304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`