| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,089,275 | T→C | H64R (CAC→CGC) | yoeB ← | ribosome‑dependent mRNA interferase toxin YoeB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,089,275 | 0 | T | C | 97.8% | 155.0 / NA | 46 | H64R (CAC→CGC) | yoeB | ribosome‑dependent mRNA interferase toxin YoeB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (30/14); minor base G (1/0); total (32/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACAATTCGCTTATCTG > NC_000913/2089142‑2089410 | aTACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGctcct > 2:430523/1‑140 (MQ=255) ttAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAAt < 2:408518/140‑1 (MQ=255) ttAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAAt > 1:346850/1‑140 (MQ=255) taAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGa > 2:99122/1‑140 (MQ=255) taAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGa > 2:381036/1‑140 (MQ=255) cGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAAc > 1:340992/1‑140 (MQ=255) cGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAAc > 1:597298/1‑140 (MQ=255) cGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAAACGAAGCTCCTCTGTAATGCGTCGGGACCAGAAAc > 2:326617/1‑140 (MQ=255) gCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGCCGGGGCTGCTCTGTAATGCGTAGGGACCAGAAACCTGAc > 2:222464/1‑140 (MQ=255) cGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACa > 2:153074/1‑140 (MQ=255) cAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTa > 1:587618/1‑140 (MQ=255) aCAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAg > 2:599191/1‑140 (MQ=255) tcattcTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGtt < 1:424047/140‑1 (MQ=255) cTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGc > 1:438625/1‑140 (MQ=255) cTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGc < 1:599191/140‑1 (MQ=255) gaatgaatCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTcc > 1:595852/1‑140 (MQ=255) tGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCtt > 1:21476/1‑140 (MQ=255) ttCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCt < 1:595751/132‑1 (MQ=255) ttCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCt > 2:595751/1‑132 (MQ=255) ttCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCa > 2:70315/1‑140 (MQ=255) ttCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCa > 2:667700/1‑140 (MQ=255) aTAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACCAATTATGTTTCAGGGGATCTGGCTTCCCCTGACCTTCAAATGGCGTTc > 2:208700/1‑140 (MQ=255) aTAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTc > 1:465276/1‑140 (MQ=255) aTAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTc > 1:378819/1‑140 (MQ=255) aTGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTa < 2:340992/140‑1 (MQ=255) cAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTg > 1:54866/1‑140 (MQ=255) cAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTg > 1:629192/1‑140 (MQ=255) aTGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGAt < 2:346121/140‑1 (MQ=255) gAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATaa < 2:346850/140‑1 (MQ=255) tGAATCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCATCCCCTTAACTTAAAATGGCGATCTGCGGGTATCTTTGATAAGTTCAt > 2:199386/1‑140 (MQ=255) aaTCGTCGGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTg < 1:470391/140‑1 (MQ=255) tcgtcgGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGAt < 1:125977/140‑1 (MQ=255) cgtcgGTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATc > 2:116627/1‑140 (MQ=255) tcgGTAACCGCGTATTCCAGACCGCTCTCGTCTGTAAGGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCtt < 1:326617/140‑1 (MQ=255) gTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCTTTCTGCGGGTAACTTTGATAAGTTCATTGATCttttt > 1:195375/1‑140 (MQ=255) gTAACCGCGTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCttttt > 2:475126/1‑140 (MQ=255) aaCCGCGTATACCAGGCGGCGCCCCCCTGCAATGCGTTGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTaa < 1:222464/140‑1 (MQ=255) ccGCGTATACCAGACGGCGCTCCTCTGTACTGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGATCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACa < 1:199386/140‑1 (MQ=255) gcgTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACAAt < 1:430523/140‑1 (MQ=255) gcgTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACAAt > 2:465482/1‑140 (MQ=255) cgTATACCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACAAtt > 2:650399/1‑140 (MQ=255) taCCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACAATTCGCt > 2:395535/1‑140 (MQ=255) taCCAGACGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGAAAAGCTCTTTGATCTTTTTAACAATTCGCt > 1:297158/1‑140 (MQ=255) ccAGACGGCGCTCCTCTATAATCCGTCGGGACCAGTAACATGACAAATCATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACAATTCGCTTa < 2:195375/140‑1 (MQ=255) aCGGCGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTTATTGATATTTTTAACAATTCGCTTATCTg > 1:228289/1‑140 (MQ=255) aCGGCGCTCCTCTGTAATCCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGTGTTCTGGCTTCCCCTTATCTTCAAATGGCGGTCTGCGGGTATCTATGTAAAGTTCATTGATTTTCTGAACAATTCGCTTATCTg > 2:271336/1‑140 (MQ=255) | ATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACAATTCGCTTATCTG > NC_000913/2089142‑2089410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |