Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,569	T→C	E136E (GAA→GAG)	rfbD ←	dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,569	0	T	C	100.0%	117.3 / NA	34	E136E (GAA→GAG)	rfbD	dTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

ATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGGATAACCCAGGCTCCAACTTCATTT  >  NC_000913/2111441‑2111701                                                                                                                                 |                                                                                                                                     ataaCCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCGGTAAAGCTTTCTCTCCGGCTaa                                                                                                                           >  2:286259/1‑140 (MQ=255)      cGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTgg                                                                                                                      >  1:372955/1‑140 (MQ=255)         tAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGttt                                                                                                                   <  2:510623/140‑1 (MQ=255)               ttcACGCTCTTTTGCCAGACGTTACTTCGCTTTGGCGCAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGt                                                                                                             <  1:216945/140‑1 (MQ=255)               ttcACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGt                                                                                                             <  2:594349/140‑1 (MQ=255)               ttcACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGt                                                                                                             <  1:373781/140‑1 (MQ=255)                 cACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTaa                                                                                                           <  1:537883/140‑1 (MQ=255)                     ctctTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACAt                                                                                                       >  2:581816/1‑140 (MQ=255)                           tGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAgtg                                                                                                 >  2:554628/1‑140 (MQ=255)                             ccAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAgtggt                                                                                               <  1:584939/140‑1 (MQ=255)                              cAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAgtggtg                                                                                              <  1:70711/140‑1 (MQ=255)                                            ttttGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGtc                                                                                >  2:356971/1‑140 (MQ=255)                                             tttGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGtct                                                                               <  1:493828/140‑1 (MQ=255)                                                    aaGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCa                                                                        <  1:556369/140‑1 (MQ=255)                                                                tttcctGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGcc                                                            <  1:54039/140‑1 (MQ=255)                                                                 ttcctGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCa                                                           >  2:54041/1‑140 (MQ=255)                                                                 ttcctGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCa                                                           >  2:464985/1‑140 (MQ=255)                                                                 ttcctGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTACATACGGCTCCAGACATGGCATATCGCCa                                                           >  1:278918/1‑140 (MQ=255)                                                                          aGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAggg                                                  <  2:106555/140‑1 (MQ=255)                                                                            aCCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAggg                                                  >  2:559435/1‑138 (MQ=255)                                                                            aCCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAggg                                                  <  1:559435/138‑1 (MQ=255)                                                                            aCCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGaa                                                >  1:343963/1‑140 (MQ=255)                                                                            aCCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCAGCCGTCTCAAGCTATAGCATATCGCCATTTCCAGGGaa                                                >  1:199080/1‑140 (MQ=255)                                                                                 gCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGt                                           >  1:332822/1‑140 (MQ=255)                                                                                  cTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTa                                          >  1:584443/1‑140 (MQ=255)                                                                                    ggTCCGGAAAATAAGATGCTTCTCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAAt                                        <  1:312123/140‑1 (MQ=255)                                                                                    ggTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAAt                                        <  2:507366/140‑1 (MQ=255)                                                                                       ccGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAg                                     >  2:282930/1‑140 (MQ=255)                                                                                                        tCGCGCAATATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGGATAAcc                    <  2:372955/140‑1 (MQ=255)                                                                                                              aaTATTCCTGTAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGGATAACCCAGGCt              <  1:554628/140‑1 (MQ=255)                                                                                                                        tAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGGATAACCCAGGCTCCAACTTCAt    >  1:460985/1‑140 (MQ=255)                                                                                                                        tAACGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGGATAACCCAGGCTCCAACTTCAt    >  1:11247/1‑140 (MQ=255)                                                                                                                          aCGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGTATAACCCAGGCTCCAACTTCAttt  >  2:434625/1‑140 (MQ=255)                                                                                                                          aCGCTTTCTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGGATAACCCAGGCTCCAACTTCAttt  >  1:443116/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                     ATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGTAATCAGTCGAGTAATGGATAACCCAGGCTCCAACTTCATTT  >  NC_000913/2111441‑2111701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑