Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,153,889	C→T	intergenic (+108/‑127)	sibB → / → mdtA	small RNA SibB/multidrug efflux pump membrane fusion protein MdtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,153,889	0	C	T	100.0%	150.5 / NA	45	intergenic (+108/‑127)	sibB/mdtA	small RNA SibB/multidrug efflux pump membrane fusion protein MdtA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/27);  total (18/27)

GGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGAAAGGTA  >  NC_000913/2153756‑2154025                                                                                                                                      |                                                                                                                                         ggCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCaa                                                                                                                                    >  1:606647/1‑140 (MQ=255)     ccTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGGTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAAtgt                                                                                                                                <  1:306059/140‑1 (MQ=255)        tCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGcc                                                                                                                             >  2:119504/1‑140 (MQ=255)                  gggCTTTCCCGTTTTAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAg                                                                                                                   >  1:547012/1‑140 (MQ=255)                   ggCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGa                                                                                                                  <  1:26342/140‑1 (MQ=255)                   ggCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGa                                                                                                                  <  2:432110/140‑1 (MQ=255)                   ggCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGa                                                                                                                  <  2:165535/140‑1 (MQ=255)                   ggCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGa                                                                                                                  <  2:646789/140‑1 (MQ=255)                   ggCTTTCCCGTTTCAGCATCCCCCTGAAATCATCGGCTTTCCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGa                                                                                                                  <  2:284234/140‑1 (MQ=21)                             tttCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACg                                                                                                                <  1:492678/132‑1 (MQ=255)                             tttCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACg                                                                                                                >  2:492678/1‑132 (MQ=255)                             tttCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGa                                                                                                        <  2:18302/140‑1 (MQ=255)                             tttCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGa                                                                                                        <  2:610851/140‑1 (MQ=255)                             tttCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGa                                                                                                        <  2:606647/140‑1 (MQ=255)                               tCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACa                                                                                                      <  2:59034/140‑1 (MQ=255)                               tCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACa                                                                                                      <  1:106476/140‑1 (MQ=255)                               tCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACa                                                                                                      <  1:659835/140‑1 (MQ=255)                               tCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACa                                                                                                      <  2:66135/140‑1 (MQ=255)                                  gCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAgtg                                                                                                   <  2:659558/140‑1 (MQ=255)                                        cGCTGAAATCGTCGGCTTACCTGCTCTCGCCATGCAACCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCAt                                                                                             <  1:275873/140‑1 (MQ=255)                                             aaaTCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATtc                                                                                        <  2:103630/140‑1 (MQ=255)                                                         tACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTtct                                                                            <  2:399727/140‑1 (MQ=255)                                                                     ccATGCAAGCAGTCTATTGCTAACGCGTAGAGAAAGTAGCTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATtggct                                                                <  2:243417/140‑1 (MQ=255)                                                                     ccATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATtggct                                                                <  2:9951/140‑1 (MQ=255)                                                                         gCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTggctggct                                                            >  2:586242/1‑140 (MQ=255)                                                                         gCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTggctggct                                                            >  2:128374/1‑140 (MQ=255)                                                                            aGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTaca                                                         >  1:337278/1‑140 (MQ=255)                                                                                        cTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCAAAATTCCTCGATTCTTCTCCCTTATTCGCTGACTACACTAGTATCATTc                                             >  2:275716/1‑140 (MQ=255)                                                                                           aCGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCcgc                                          >  1:383980/1‑140 (MQ=255)                                                                                             gcgTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGa                                        >  2:509598/1‑140 (MQ=255)                                                                                                     aaaaTAGTGTCCTGTGCTATTAGTGGATGGGCGTTCGCATATGTTCCCATCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAACCGTTTc                                <  2:323028/140‑1 (MQ=255)                                                                                                        aTAGTTTCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAgg                             <  1:7506/140‑1 (MQ=255)                                                                                                            tttCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGaa                                       <  2:604958/126‑1 (MQ=255)                                                                                                            tttCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGaa                                       >  1:604958/1‑126 (MQ=255)                                                                                                             ttCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTTCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCCTATTGGCTGGCTGCACCAGTATCATTCCGCTAAACGTTTCAGGAagag                        >  2:233622/1‑140 (MQ=255)                                                                                                             ttCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGTTACACTAGTATGATCCCGCGAGACGTTTCAGGAagag                        >  1:278026/1‑140 (MQ=255)                                                                                                             ttCCTGTGTTATTACTGGATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAagag                        >  2:648024/1‑140 (MQ=255)                                                                                                                             ggATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTTCGCGAAACGTTGCAAGAAGAGAAACTCTTAACGATGa        >  1:296106/1‑140 (MQ=255)                                                                                                                              gATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGaa       <  2:337278/140‑1 (MQ=255)                                                                                                                              gATGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGaa       <  1:648024/140‑1 (MQ=255)                                                                                                                               aTGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGaaa      >  2:556122/1‑140 (MQ=255)                                                                                                                                tGCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGAAAg     >  2:442699/1‑140 (MQ=255)                                                                                                                                 gCGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGAAAgg    <  1:509598/140‑1 (MQ=255)                                                                                                                                 gCGTGTTCGCAAATGCGCCCGTTATTCAGACGATTCCCGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGAAAgg    <  1:254591/140‑1 (MQ=255)                                                                                                                                  cGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGGGAACCGTTTCAGGAAGGGAAACTCTTAACGATGAAAGGt   >  2:254591/1‑140 (MQ=255)                                                                                                                                  cGTGTTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGAAAGGt   <  1:442699/140‑1 (MQ=255)                                                                                                                                   gtgtTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGAAAGGTa  <  2:547012/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGATAAAATAGTTTCCTGTGTTATTACTGGATGCGTGCTCGCAAATGTGCCCGTCATTCAGACGATTCCAGACAGTGTTTCATAATTCCTCCATTTTTCTCCCTTATTGGCTGGCTACACTAGTATCATTCCGCGAAACGTTTCAGGAAGAGAAACTCTTAACGATGAAAGGTA  >  NC_000913/2153756‑2154025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑