Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,803	T→C	pseudogene (476/1358 nt)	gatC ←	galactitol‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,803	0	T	C	100.0%	105.3 / NA	30	pseudogene (476/1358 nt)	gatC	galactitol‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

TAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCG  >  NC_000913/2173666‑2173930                                                                                                                                          |                                                                                                                                tAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCta                                                                                                                               <  1:69210/140‑1 (MQ=255)               ttCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAAcgccgc                                                                                                                 <  1:441049/140‑1 (MQ=255)                                gACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTg                                                                                                >  1:527185/1‑140 (MQ=255)                                 cccAGAACAGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATCCCTTCCAGCTCAAAGAAATTTCGGGTACCGCGGGTAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGc                                                                                               <  2:135449/139‑1 (MQ=255)                                 aCCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGc                                                                                               <  2:369901/140‑1 (MQ=255)                                    aGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCAt                                                                                            <  2:10967/140‑1 (MQ=255)                                      cACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATcc                                                                                          >  2:81623/1‑140 (MQ=255)                                           cAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTatc                                                                                     <  2:638762/140‑1 (MQ=255)                                           cAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTatc                                                                                     >  2:345765/1‑140 (MQ=255)                                           cAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTatc                                                                                     >  2:139190/1‑140 (MQ=255)                                                  ccccATATACGCCGAAGTACCGGGCGGAATAGCAATAACTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATg                                                                              <  1:265707/140‑1 (MQ=255)                                                               gACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTatcatc                                                                                  <  2:78315/123‑1 (MQ=255)                                                               gACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTatcatc                                                                                  >  1:78315/1‑123 (MQ=255)                                                                        tGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCaa                                                        >  2:545500/1‑140 (MQ=255)                                                                          cGGAATAGCAATACCATCCAGCCCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAAcg                                                      <  1:290959/140‑1 (MQ=255)                                                                                  cAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTg                                              >  2:510756/1‑140 (MQ=255)                                                                                  cAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCAATCCCTATAATCCACGAACCGGTTGCCAGAAGCAGCAACGCGCCGGTg                                              >  2:290959/1‑140 (MQ=255)                                                                                       ccTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGt                                         >  1:78091/1‑140 (MQ=255)                                                                                       ccTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGt                                         >  1:659075/1‑140 (MQ=255)                                                                                       ccTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGt                                         <  1:510756/140‑1 (MQ=255)                                                                                       ccTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTAGCCAGATGCAGCATCGCGCCGGTGAAGGt                                         >  1:279800/1‑140 (MQ=255)                                                                                       ccTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATCAACCGGTTGCCCGAAGCAGCAGCGCGCCGGTGAAGGt                                         >  1:270174/1‑140 (MQ=255)                                                                                       ccTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATCAACCGGTAGCCCGAGGCAGCAACGCGCCGGTGAAGGt                                         >  1:288580/1‑140 (MQ=255)                                                                                         ttCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGACCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCa                                       >  2:428743/1‑140 (MQ=255)                                                                                               cTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCatatccc                                 <  1:345765/140‑4 (MQ=255)                                                                                               cTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGcc                                 <  1:139190/140‑1 (MQ=255)                                                                                                 cAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAg                               <  2:527185/140‑1 (MQ=255)                                                                                                                             ccAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCAccc   <  2:1629/140‑1 (MQ=255)                                                                                                                              cAGTCGCCGAGCCTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCcg  <  2:659075/140‑1 (MQ=255)                                                                                                                              cAGTCGCCGAGCCTATAAACAAACGCCGCGTAAATTACCCCACCTGCCCTCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCcg  <  2:270174/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                TAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCG  >  NC_000913/2173666‑2173930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑