Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	189,107	A→G	Y134H (TAC→CAC)	map ←	methionine aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	189,107	0	A	G	100.0%	155.1 / NA	45	Y134H (TAC→CAC)	map	methionine aminopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/23);  total (22/23)

GCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTACAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCC  >  NC_000913/188979‑189236                                                                                                                                 |                                                                                                                                  gCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCtt                                                                                                                        >  2:332018/1‑140 (MQ=255)  ccGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACAATGTGTAGCGCCACGTGCAGGCTTTCTTg                                                                                                                       >  1:269398/1‑140 (MQ=255)         cAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATg                                                                                                                <  1:645355/140‑1 (MQ=255)         cAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATg                                                                                                                <  1:490931/140‑1 (MQ=255)                 tgtCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAg                                                                                                        >  2:612593/1‑140 (MQ=255)                 tgtCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAg                                                                                                        >  2:448443/1‑140 (MQ=255)                 tgtCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAg                                                                                                        >  2:30189/1‑140 (MQ=255)                   tCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGAc                                                                                                      >  1:21571/1‑140 (MQ=255)                   tCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGAc                                                                                                      >  1:342474/1‑140 (MQ=255)                    ccGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACg                                                                                                     <  1:449359/140‑1 (MQ=255)                     cGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGt                                                                                                    <  1:332018/140‑1 (MQ=255)                      gCAATATTCACGAACGACGGAGGAGCCTTCTTCTTTGACTAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGtt                                                                                                   <  2:225611/140‑1 (MQ=255)                      gCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCCTGCGTAGCGCCAGGGGCAGGCTTTCTTGCGCGATGCGGCCCAGACGtt                                                                                                   >  2:309090/1‑140 (MQ=255)                       cAATATTCACGAACGACGGAGATGCCTTCTGCTTCAACATATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTc                                                                                                  <  2:278052/140‑1 (MQ=255)                        aaTATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCg                                                                                                 >  1:457738/1‑140 (MQ=255)                        aaTATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTCCTTGCGTGATGCGGCACAGACGTTCg                                                                                                 <  1:566772/140‑1 (MQ=255)                                cgaacgaCGGAGAAGTCTTCTGATTAGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCatgat                                                                                         <  1:309090/140‑1 (MQ=255)                                cgaacgaCGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGTTTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCatgat                                                                                         <  2:78430/140‑1 (MQ=255)                                      acgGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCgg                                                                                   >  2:505190/1‑140 (MQ=255)                                                  tCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGAt                                                                       >  1:620090/1‑140 (MQ=255)                                                  tCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGAt                                                                       <  1:506929/140‑1 (MQ=255)                                                    tGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCATGATGCGGTACAGACGATCGCCCATGATGGACGGCTTACCGACGATaa                                                                     >  2:191824/1‑140 (MQ=255)                                                         cGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATTCGGCACAGACGTCCGCCCATGATGGGCGGCTTACCGACGATAAACAtt                                                                >  2:232979/1‑140 (MQ=255)                                                           aCAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACAtttt                                                              <  1:495638/140‑1 (MQ=255)                                                             aaaTTTCTGAATCGCTGCACCGATTACGCGCAGATTATTGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCg                                                            <  1:311621/140‑1 (MQ=255)                                                             aaaTTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCg                                                            <  2:342474/140‑1 (MQ=255)                                                                        tCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCg                                                            >  2:470394/1‑129 (MQ=255)                                                                        tCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCg                                                            <  1:470394/129‑1 (MQ=255)                                                                        tCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCg                                                 <  1:615237/140‑1 (MQ=255)                                                                          gccgcACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTg                                               <  1:58184/140‑1 (MQ=255)                                                                               aCCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAAc                                          <  1:578858/140‑1 (MQ=255)                                                                               aCCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAAc                                          <  1:549459/140‑1 (MQ=255)                                                                                              aTTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACgg                           <  1:30189/140‑1 (MQ=255)                                                                                                 aaTGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGTGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGa                        >  2:598260/1‑140 (MQ=255)                                                                                                  aTGCCTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGAc                       <  2:546625/140‑1 (MQ=255)                                                                                                      cTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCa                   <  2:457738/140‑1 (MQ=255)                                                                                                      cTGGTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCa                   <  2:556932/140‑1 (MQ=255)                                                                                                        ggTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAAt                 >  1:375689/1‑140 (MQ=255)                                                                                                        ggTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAAt                 >  1:435350/1‑140 (MQ=255)                                                                                                        ggTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAAt                 >  2:530205/1‑140 (MQ=255)                                                                                                        ggTTTTACCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGAGGAAACCAACTTTAATTACGGTGACATCAAt                 >  2:259747/1‑140 (MQ=255)                                                                                                              aCCATGCGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGAGGAAACCCTCTGTGATTACGGCGACATCAATGTTAAc           >  1:305848/1‑140 (MQ=255)                                                                                                                    cGTAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATc     >  1:7422/1‑140 (MQ=255)                                                                                                                      tAGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGc   <  1:530205/140‑1 (MQ=255)                                                                                                                       aGCGCCAGGTGCAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGcc  >  2:476220/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                  GCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGCCGCACCGATTTCGCGCAGATTAATGCCTGGTTTTACCATGCGTAGCGCCAGGTACAGGCTTTCTTGCGTGATGCGGCACAGACGTTCGCCCATGATGGTCGGCTTACCGACGATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCC  >  NC_000913/188979‑189236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑