| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,272,277 | T→C | W518R (TGG→CGG) | pdeN → | putative c‑di‑GMP phosphodiesterase PdeN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,272,277 | 0 | T | C | 96.0% | 74.4 / ‑4.0 | 25 | W518R (TGG→CGG) | pdeN | putative c‑di‑GMP phosphodiesterase PdeN |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (8/16); total (8/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
TGCTGAAGGGGTCGAAACGCCGGAACAGGCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGTGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAGATGATTGTGCGCATACTGCTGCTGTTTATCGCTCTGTTCACCTTTG > NC_000913/2272138‑2272409 | tGCTGAAGGGGTCGAAACGCCGGAACAGGCGCGATGGCTAAGCGAACGCGGAGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGAATTTGTTCTCTGCCTCAAGAACCCGTATACGCCGCAGt > 1:328923/1‑140 (MQ=255) aGGGGTCGAAACGCCGGAACAGGCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAg < 1:605600/140‑1 (MQ=255) ggTCGAAACGCCGGAACAGGCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGgtg < 1:362773/140‑1 (MQ=255) ggTCGAAACGCCGGAACAGGCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGATGGCTAAGGAATCCATATACGTCGCAGCGGTAAGgtg > 1:264585/1‑140 (MQ=255) aCGCCGGAACAGGCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTAc < 2:461776/140‑1 (MQ=255) ccGGAACAGGCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGTGGTAAGGTGTGCTTACGTc < 2:124466/140‑1 (MQ=255) ccGGAACAGGCGCGATGCCTAAGCGAACGCGTCGTGAATCTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTc < 2:251995/140‑1 (MQ=255) cGGAACAGGCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTcc > 2:559225/1‑140 (MQ=255) aCAGGCGCGATGGCTAAGCGAACGTGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCtta < 2:31681/140‑1 (MQ=255) cAGGCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCttat < 2:106517/140‑1 (MQ=255) gcgATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTc < 1:571650/140‑1 (MQ=255) aaGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGaaa < 1:572290/140‑1 (MQ=255) cgcgGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGcc > 1:43444/1‑140 (MQ=255) gcgTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCGTACGTCCCTTAGTAATCATAGTGAAAGCATGCCGGAt > 1:324583/1‑140 (MQ=255) tGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAgg < 2:359441/140‑1 (MQ=255) gccgccCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCg < 1:611446/140‑1 (MQ=255) gccgccCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCAGAGTGAAAGGATGGCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCg > 2:229804/1‑140 (MQ=255) ccGCTGGATGATTTTGTACGCTGGCTAAAGCGACCGTATGCGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAgatgat < 2:324583/140‑1 (MQ=255) ccGCTGGACGATTTTGTTCGCTTGCTAAAGATATCGTATGCCCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAgatgat < 2:303694/140‑1 (MQ=255) cTGGACGATTTTGTTCGCTGGCTAATGAATCCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAGATGATtgt < 1:229804/140‑1 (MQ=255) gACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAGATGATTGTgcg > 1:642476/1‑140 (MQ=255) cGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAGATGATTGTGCGCa < 2:642476/140‑1 (MQ=255) tGGCTAAAGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAGTTGATTGTGCACATACTGCTGCTGtt > 1:286771/1‑140 (MQ=255) aaaGAAACCGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAGATGATTGTGCGCATACTGCTGCTGTTTATCg < 1:459676/140‑1 (MQ=255) ccGTATACGCCGCAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAGATGATTGTGCGCATACTGCTGCTGTTTATCGCTCTGtt < 1:559225/140‑1 (MQ=255) gccgcAGCGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAGATGATTGTGCGCATACTGCTGCTGTTTATCGCTCTGTTCACCTTTg > 2:61921/1‑140 (MQ=255) | TGCTGAAGGGGTCGAAACGCCGGAACAGGCGCGATGGCTAAGCGAACGCGGCGTTAATTTCATGCAAGGCTACTGGATTAGCCGCCCGTTACCGCTGGACGATTTTGTTCGCTGGCTAAAGAAACCGTATACGCCGCAGTGGTAAGGTGTGCTTACGTCCCTTATTATTCATAGTGAAAGCATGCCGGATTGCGGCTAATGATGAGTAAAAGGAAATCCGTTGCAGATGATTGTGCGCATACTGCTGCTGTTTATCGCTCTGTTCACCTTTG > NC_000913/2272138‑2272409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |