Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,302,553	A→G	C67C (TGT→TGC)	napA ←	periplasmic nitrate reductase subunit NapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,302,553	0	A	G	100.0%	73.2 / NA	22	C67C (TGT→TGC)	napA	periplasmic nitrate reductase subunit NapA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

CCTTCTTTGTCATATTTACCGTTTTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCA  >  NC_000913/2302416‑2302680                                                                                                                                          |                                                                                                                                ccTTCTTTGTCATATTTACCGTTTTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCa                                                                                                                               >  2:329230/1‑140 (MQ=255)      tttGTCATGTTTACCGTGTTTCTTACGCAGCTGTGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGcca                                                                                                                          <  2:326987/140‑1 (MQ=255)      tttGTCATATTTACCGTTTTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGcca                                                                                                                          <  1:459581/140‑1 (MQ=255)           cATATTTACCGTTTTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACcaca                                                                                                                     <  2:77278/140‑1 (MQ=255)           cATATTTACCGTTTTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACcaca                                                                                                                     <  2:451881/140‑1 (MQ=255)                  aCCGTTTTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACcaca                                                                                                                     <  2:375814/133‑1 (MQ=255)                  aCCGTTTTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACcaca                                                                                                                     >  1:375814/1‑133 (MQ=255)                                        ggCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAAcgcc                                                                                        <  1:646027/140‑1 (MQ=255)                                                           tACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGaaa                                                                     <  2:537328/140‑1 (MQ=255)                                                               gTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAacggc                                                                 >  1:633762/1‑140 (MQ=255)                                                                 aCATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAacggcac                                                               >  1:474724/1‑140 (MQ=255)                                                                     gATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGgcg                                                           >  1:625713/1‑140 (MQ=255)                                                                            ggCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATc                                                    >  1:78130/1‑140 (MQ=255)                                                                             gCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATcc                                                   <  2:633762/140‑1 (MQ=255)                                                                                         ccTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCt                                       <  1:482619/140‑1 (MQ=255)                                                                                         ccTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCt                                       <  2:625713/140‑1 (MQ=255)                                                                                              aTGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCctg                                  >  2:550848/1‑140 (MQ=255)                                                                                                gCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCctgct                                >  1:84227/1‑140 (MQ=255)                                                                                                 cAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGGACCGCAGAGACGGCACGGCGCTTTATCCCAATTGATGGCTTCctgctg                               >  2:215249/1‑140 (MQ=255)                                                                                                              ggTTAACCGGTGCGTCCGGGTCTCCCTGGCACGCCCCCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGgcg                  <  2:235161/140‑1 (MQ=255)                                                                                                                            tCCGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCgg    <  1:550848/140‑1 (MQ=255)                                                                                                                              cGGGTCGCCCTGGCAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCa  >  2:95233/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                CCTTCTTTGTCATATTTACCGTTTTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCA  >  NC_000913/2302416‑2302680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑