Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,310,285	A→G	S41P (TCT→CCT)	ada ←	DNA‑binding transcriptional dual regulator/DNA repair protein Ada

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,310,285	0	A	G	100.0%	75.9 / NA	22	S41P (TCT→CCT)	ada	DNA‑binding transcriptional dual regulator/DNA repair protein Ada
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

TTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTT  >  NC_000913/2310149‑2310421                                                                                                                                         |                                                                                                                                         ttATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAggcgg                                                                                                                                       >  2:657147/1‑140 (MQ=255)     ccAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATCTCTGACGCGGCAAGgcggacgg                                                                                                                                   >  2:284334/1‑140 (MQ=255)         ccGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCaaa                                                                                                                               >  1:475684/1‑140 (MQ=255)         ccGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCaaa                                                                                                                               >  2:111916/1‑140 (MQ=255)            aTGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGa                                                                                                                            <  2:357203/140‑1 (MQ=255)               ttGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGCGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGc                                                                                                                         <  1:469974/140‑1 (MQ=255)                 gCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCt                                                                                                                       <  1:503072/140‑1 (MQ=255)                       cATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCTGTGGTa                                                                                                                 >  2:154918/1‑140 (MQ=255)                          tGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGc                                                                                                              >  1:391046/1‑140 (MQ=255)                                                         gCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCTGTCGTACGCAAGGCTAAGACGAATTCGCCGTCGGCATTCg                                                                               >  1:222394/1‑140 (MQ=255)                                                              aGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcg                                                                          <  2:17345/140‑1 (MQ=255)                                                              aGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcg                                                                          <  2:97708/140‑1 (MQ=255)                                                                      gcgAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTaa                                                                  <  2:391046/140‑1 (MQ=255)                                                                                     gCATTTGCGTAGAAGTAGACGTTTTGCCGCAAAGCATTTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCg                                                   <  1:284334/140‑1 (MQ=255)                                                                                      cATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGt                                                  >  2:654397/1‑140 (MQ=255)                                                                                        tttGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTg                                                <  1:654397/140‑1 (MQ=255)                                                                                                                               gcgcGGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTc         >  2:20918/1‑140 (MQ=255)                                                                                                                                cgcgGCAAGGCGGACGGGAAAAGATGCCTGTTGTACGCACGGCGAAAAGGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTcc        <  2:289693/140‑1 (MQ=255)                                                                                                                                cgcgGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTcc        <  1:20918/140‑1 (MQ=255)                                                                                                                                 gcgGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTccc       <  2:475684/140‑1 (MQ=255)                                                                                                                                 gcgGCAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTccc       >  2:650068/1‑140 (MQ=255)                                                                                                                                     cAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGt   >  1:356090/1‑140 (MQ=255)                                                                                                                                     cAAGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGt   <  2:653142/140‑1 (MQ=255)                                                                                                                                      aaGGCGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGtt  <  1:111916/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTATCCAACCGATGTTGCTGGGCATTGGCTTTTTCTGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTT  >  NC_000913/2310149‑2310421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑